金莲花药材的质量标准研究  

Study on the quality standard of Trollius chinensis

作　　者：宋联强 汤燕[2] 詹雁[2] 谭镭[2] 舒光明[2] 袁志翔[2] 徐超群[2] 

机构地区：[1]成都中医药大学药学院,四川成都610075 [2]四川省中医药科学院,四川成都610041

出　　处：《华西药学杂志》2015年第4期484-486,共3页West China Journal of Pharmaceutical Sciences

基　　金：中国药典标准(2015年版起草研究);四川省公益性科研所基本科研业务专项

摘　　要：目的研究并建立金莲花药材的质量标准。方法采用性状描述、显微鉴别、有效成分定性和定量分析等方法控制金莲花药材的质量。结果金莲花药材粉末的显微鉴别特征性强,薄层色谱的斑点清晰,定量分析采用HPLC方法同时测定了荭草素和荭草素-2"-O-β-L-半乳糖苷,平均加样回收率分别为98.42%(RSD=0.82%)、100.87%(RSD=0.86%),10批药材中二者的平均含量分别为1.31%、0.48%,并制定了二者总含量的限度。结论所用方法简便、可靠、稳定、准确,能全面控制金莲花药材的质量。OBJECTIVE To study and establish the quality standard of Trollius chinensis. METHODS Morphological, pharmacog- nostic, effective components qualitative and quantitative analysis methods were used to control the medicinal materials quality of T. chinensis. RESULTS The morphological and characteristic microscopic were specific. TLC spots were clear. The average content of orientin and 2＂ -O-β -L- galactopyranosyl orientin in 10 batches of herbs were 1.31% and 0.48% ,and the limit of the total content was set. And the recoveries were 98. 42% and 100. 87% with the RSD of 0. 82% and 0. 86% （ n = 6） , respectively. CONCLUSION The methods is specific,simple,reliable and can be used for the quality control of T. chinensis.

关 键 词：金莲花 荭草素 荭草素-2”-O-β-L-半乳糖苷 质量标准 性状描述 生药学鉴别 有效成分 定性分析 定量分析 薄层色谱法 

分 类 号：R917[医药卫生—药物分析学]