低氧对新生隐球菌荚膜厚度的影响  

Effects of hypoxia on the capsule size of Cryptococcus neoformans

作　　者：杜雪[1] 孔庆涛[1] 黄苏扬 杨瑞[1] 桑红[1] 

机构地区：[1]南京大学医学院附属金陵医院(南京军区南京总医院)皮肤科,南京医学硕士研究生210002

出　　处：《医学研究生学报》2015年第8期799-803,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81371782)

摘　　要：目的低氧对新生隐球菌荚膜形成的影响尚不清楚。文中旨在研究低氧条件对新生隐球菌荚膜的影响。方法使用液体沙氏培养基培养39株新生隐球菌临床分离株及标准株,以三气培养箱建立正常氧浓度(21%)和低氧浓度(1%)。将培养的菌株分别置于不同氧浓度的培养箱中培养,借助显微镜观察并通过软件测量不同氧浓度对新生隐球菌荚膜厚度的影响。结果 39株受试菌株中,30株菌株的荚膜厚度在正常氧浓度下较低氧浓度下增加,其中17株菌株厚度差异具有统计学意义(P<0.05)。9株菌株荚膜厚度在低氧浓度下较正常氧浓度下减小,其中4株菌株厚度差异具有统计学意义(P<0.05)。结论低氧有抑制新生隐球菌荚膜生成的倾向,但是否与菌株特性如血清型、基因型有关仍需进一步研究。Objective Little is known about the role of low oxygen concentration in the formation of the capsule of Cryptococ- cus neoformans. This study was to investigate the effect of hypoxic condition on the capsule size of Cryptococcus neoformans. Methods We cultured 39 Cryptococcus neoformans strains in liquid Sabouraud medium and then incubated them at the normoxic （21%） and hypoxic concentration （1%）, respectively. After 72 hours of incubation, we observed the capsules formed at different ox- ygen conditions under the Olympus microscope and the measured their sizes using the Axiovision software. Results Of the total number of Cryptococcus neoformans strains, 30 showed larger capsules in the normoxic than in the hypoxic condition, 17 with statisti- cally significant differences in their diameters （P 〈 0.05）, while the other 9 strains exhibited just the opposite results, g with statisti- cally significant differences in their diameters （ P 〈 0.05 ）. Conclusion Hypoxia might inhibit the growth of the Cryptococcus neoformans capsule. However, further studies are needed on its correlation with the serum or gene types of the Cryptococcus neofor- mans strain.

关 键 词：新生隐球菌 氧浓度 荚膜 

分 类 号：R379.5[医药卫生—病原生物学]