缺氧预处理对颅脑损伤周围皮质神经血管单元的影响  

Effects of hypoxic preconditioning on neurovascular unit around cortex after traumatic brain injury in rats

作　　者：卓健伟 刘家传[1] 杨艳艳[1] 王金标[1] 张永明[1] 张星[1] 孙文江[1] 

机构地区：[1]解放军105医院神经外科,合肥230031

出　　处：《中国微侵袭神经外科杂志》2015年第8期375-378,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：全军医学科技"十二五"科研项目(编号:CWS11J262);2009年度南京军区医学科技创新重点课题(编号:09Z009)

摘　　要：目的观察缺氧预处理对颅脑损伤周围皮质神经血管单元(NVU)的影响。方法 108只SD雄性大鼠随机分为对照组(n=6)、缺氧预处理组(HPC组n=6)、颅脑损伤组(TBI组n=48)、缺氧预处理+颅脑损伤组(HPCT组n=48)。免疫组化技术检测神经元核抗原(Neu N)、胶质纤维酸性蛋白(GFAP),Ig G法测定血-脑屏障通透性变化情况。结果 TBI组和HPCT组的GFAP在伤后3 h^14 d、Ig G在伤后1 h^14 d均增加,而Neu N在两组表达分别在伤后3 h^14 d、6 h^14 d减少(均P<0.05)。HPCT组Neu N在伤后3 h^7 d均多于TBI组,GFAP在伤后6 h^1 d多于TBI组而伤后7 d少于TBI组,Ig G在伤后1 h^14 d少于TBI组(P<0.05)。结论缺氧预处理在一定时间窗内保护神经元,调整星形胶质细胞活化,降低血-脑屏障通透性,减轻随后颅脑损伤对周围皮质NVU的损伤。Objective To observe the influence ofhypoxic preconditioning on neurovascular unit （NVU） arourid cortex after traumatic brain injury in rats. Methods One hundred and eight SD male rats were randomly divided into control group （n = 6）, hypoxic preconditioning group （HPC, n = 6）, traumatic brain injury group （TBI, n = 48） and traumatic brain injury group after hypoxic preconditioning （HPCT, n = 48）. Immunohistochemistry method was used to measure the expression of neural nuclei （NeuN） and glial fibrillary acidic protein （GFAP）. The IgG was used to represent the change of blood-brain barrier permeability. Results GFAP expression at 3 h-14 d after injury and IgG expression at 1 h-14 d after injury all significantly increased in TBI group and HPCT group. The expression of NeuN significantly decreased at 3 h-14 d after injury in TBI group, while significantly decreased at 6 h-14 d after injury in HPCT group （all P 〈 0.05）. Compared with TBI group, NeuN expression at 3 h-7 d after injury was higher in I-IPCT group. GFAP expression at 6 h-I d after injury was higher in HPCT group than in TBI group, conversely, the expression at 7 d after injury was lower. And IgG expression at 1 h-14 d after injury was lower in HPCT group than in TBI group （P〈 0.05）. Conclusions Within a effective time window, hypoxic preconditioning can protect neurons, adjust astroglia＇s activiation, and reduce the blood-brain barrier permeability. Thereby hypoxic preconditioning has the function of reducing the following damage of NVU in the surrounding cortex after TBI.

关 键 词：颅脑损伤 缺氧预处理 神经血管单元 

分 类 号：R651.15[医药卫生—外科学]