广西汉族人群NPC1L1基因1735C>G多态性分布特征及与血脂、肥胖的关系  

Distribution characteristics of NPC1L1 gene 1735 C>G polymorphism and relationship between serum lipid levels and obesity in Guangxi Han population

作　　者：苗林[1] 尹瑞兴[2] 吴东峰[3] 

机构地区：[1]广西医科大学第四附属医院心内科,广西柳州545005 [2]广西医科大学第一附属医院心内科,南宁530021 [3]广西壮族自治区人民医院综合干部病区,南宁530021

出　　处：《重庆医学》2015年第24期3341-3344,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30960130)

摘　　要：目的分析NPC1L1基因1 735C>G多态性分布特征及其与血脂、肥胖的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对409例汉族人群进行NPC1L1基因多态性分型,比较不同基因型间血脂水平、BMI的差异。结果 CC,CG,GG基因型的分布频率分别为39.40%、46.00%、14.60%;等位基因C和G频率分别为62.35%和37.65%。3种基因型组间的LDL-C、ApoB水平和BMI的差异有统计学意义(P<0.05),G等位基因携带者(GC/GG基因型)较CC型有更高的LDL-C、ApoB水平(P<0.05)。血脂谱和BMI与多种环境因素相关。结论 NPC1L1基因1 735C>G多态性与血脂水平和BMI相关,G等位基因可能是高LDL-C水平和肥胖的一个危险因素。Objective To analyze the distributional characteristics of NPCIL1 1 735 C〈G polymorphism and the relation- ship between serum lipid levels and obesity in Guangxi Han population. Methods Genotyping of the NPC1L1 1 735 C〉G poly- morphism of 409 cases of Han was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis in all subjects. The relationship of different genotypes in blood lipid levels and obesity were evaluated. Re- suits The frequency of CC,CG and GG genotypes was 39.40% ,46.00% and 14.60% in Han. The frequency of C and G alleles was 62.35% and 37.65% in Han. The levels of BMI,LDL-C,ApoB was different among the three genotypes （P〈0.05）. The G al- lele carriers had higher serum LDL-C and ApoB levels than the G allele noncarriers （P〈0.05）. Serum lipid parameters and BMI were also correlated with some environmental factors. Conclusion The 1 735 C〈G polymorphism in NPC1L1 gene may be correla- ted with serum lipid profiles and obesity,the G allele might increase the risk of hyperlipemia and obesity.

关 键 词：多态性 单核苷酸 血脂异常 NPC1L1 人体质量指数 

分 类 号：R589.2[医药卫生—内分泌]