兔解偶联蛋白1基因第2外显子基因多态性研究  

Study on the SNP of UCP1 Gene Exon 2 in Rabbit

作　　者：李维[1] 刘若余[1] 林家栋[1] 谢海强[1] 李思[1] 杜雪琴 肖超能[1] 

机构地区：[1]贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [2]贵阳市小河第三实验学校,贵阳550006

出　　处：《中国畜牧兽医》2015年第8期2131-2136,共6页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省科技支撑计划项目(农业攻关,黔科合NY字[2012]3013号)

摘　　要：为了解兔肉质性状特征,给兔的品种选种选育提供理论依据。本试验选取新西兰兔和加利福尼亚兔分别构建各自的DNA池,对解偶联蛋白1(uncouplingprotein 1,UCP1)基因的第2外显子及第1内含子部分序列进行扩增,扩增产物进行双向测序,利用BLAST和DNAStar分析并确定其SNP。结果分析,在新西兰兔中发现6个SNPs:T135C、C232T、T332C、C360T、T550C和A560G,其中T135C和C232T为同义突变,T332C、C360T、T550C、A560G4个突变位点位于内含子区。利用RNA在线预测软件预测UCP1基因RNA二级结构最小自由能由-917.51kJ/mol变为-866.10kJ/mol,说明SNPs对UCP1基因的RNA二级结构产生影响。In order to deeply understand the meat quality and provide the basic information and scientific proof for better heredity selection of rabbit. This experiment option New Zealand rabbit and Californian rabbit were used to construct own DNA pools,respectively for complete sequences amplification of exon 2 and part of intron 1 region of UCP 1 gene, two-way sequencing the amplifi- cation of the product, BLAST and DNAStar analysis and determine the SNP of rabbit UCP1 gene. The results showed that six SNPs were found in UCP1 gene of the New Zealand rabbit. T135C, C232T, T332C, C360T, T550C and A560G, two synonymous mutation （T135C and C232T） and the other SNPs located in intron region respectively. Using RNA online prediction software forecast UCP1 gene RNA secondary structure prediction software minimum free energy changed from --917.51 kJ/mol to --866.10 kJ/mol for the polymorphisms led to the changes of RNA secondary structure.

关 键 词：肥胖 UCP1基因 新西兰兔 SNPS 加利福尼亚兔 

分 类 号：S813.3[农业科学—畜牧学]