毒害艾美耳球虫感染对雏鸡肠道组织热休克蛋白-90 mRNA表达的影响  

Effect of Heat Shock Protein-90(HSP-90) mRNA Expression in Intestinal Tissuse of Chickens Infected with E.necatrix

作　　者：侯宁[1] 吕志慧[2] 赵靓[1] 高雪丽[1] 刘超男[1] 吕晓萍[1] 郑世民[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]遵义医学院珠海校区,珠海519041

出　　处：《中国畜牧兽医》2015年第8期2183-2188,共6页China Animal Husbandry & Veterinary Medicine

基　　金：黑龙江省教育厅项目(12531015)

摘　　要：将90只海蓝褐雏鸡随机分为对照组、毒害艾美耳球虫（E.necatrix）一次感染组和毒害艾美耳球虫两次感染组,应用二重PCR方法,通过对肠道组织热休克蛋白-90（HSP-90）mRNA表达的检测,较全面系统地研究了毒害艾美耳球虫感染对雏鸡肠道组织HSP-90 mRNA表达的影响。结果发现,14日龄雏鸡经口第一次感染毒害艾美耳球虫后0~14d,其肠道组织HSP-90mRNA表达虽然不同程度的低于对照雏鸡,但总体呈上升趋势;上述雏鸡于28日龄时再次攻击性感染毒害艾美耳球虫,其十二指肠、空肠和回肠HSP-90 mRNA表达均呈先下降后上升,而盲肠HSP-90 mRNA表达始终呈下降趋势。28日龄雏鸡一次性大剂量感染毒害艾美耳球虫后,其十二指肠、空肠、盲肠HSP-90 mRNA表达均下降,而回肠HSP-90 mRNA表达先下降后上升。结果表明毒害艾美耳球虫感染雏鸡初期,肠道组织HSP-90 mRNA表达受到一定抑制,其表达量与肠道组织细胞损伤程度密切相关。The 90 chickens were randomly divided into control group,E, necatrix one time infec- tion group and E. necatrix two times infection group. We used duplex PCR method to test the ex- pression changes of HSP-90 mRNA in intestinal tissue of chickens infected with E. necatrix and researched the effects of it comprehensively and systematically. The results were showed that af- ter 0 to 14 days the expression of HSP-90 mRNA in intestinal tissues of 14-day-old chickens infec- ted with E. necatrix presented a upward trend though it was lower than the control chickens. These chickens were infected with E. necatrix again at 28 days, HSP-90 mRNA expressions of du- odenum,jejunum and ileum decreased and then increased. However,it always showed a downward trend in appendix. When 28 days chickens were infected with high-does E. necatrix once, HSP-90 mRNA expressions of duodenum,jejunum and appendix were in a declining curve. While it was in- creased originally and then decreased in ileum. This study indicated that HSP-90 mRNA expres- sions in intestinal tissue of chickens infected with E. necatrix were inhibited at first, and the ex- pression quantity was closely related to the degrees of intestinal tissue damage.

关 键 词：毒害艾美耳球虫 雏鸡 肠道组织 HSP-90 MRNA表达 

分 类 号：Q786[生物学—分子生物学]