CD4^+CD25^+Foxp3^+Treg与子宫内膜异位症的相关性探讨  

Correlation between CD4^+CD25^+Foxp3^+Treg and endometriosis

作　　者：夏震[1] 蔡海莉[1] 刘瑞霞[1] 王晓兰[1] 

机构地区：[1]暨南大学医学院附属第二医院(广东省深圳市人民医院)妇产科,广东深圳518000

出　　处：《中国妇幼保健》2015年第24期4223-4225,共3页Maternal and Child Health Care of China

摘　　要：目的通过使用流式细胞术对子宫内膜异位症(EM)患者外周血单个核细胞(PBMC)中CD4+ CD25+叉头蛋白3(Foxp3+)调节性T细胞(Treg)所占比例的检测,探讨EM与Treg的关系。方法分析2012年8月~2014年4月在该院接受治疗的EM患者(观察组)的临床资料。选择同期经门诊体检无EM的健康的非孕期、非月经期的女性志愿者作为对照组。比较两组受试者以及EM患者不同分期所对应的外周血CD4+CD25+Foxp3+Treg绝对数和在CD4+T淋巴细胞所占比例。结果该研究共纳入受试者78例,其中观察组患者45例,对照组33例。观察组患者的CD4+CD25+Foxp3+、Treg的绝对数量和所占CD4+T淋巴细胞比例均显著高于对照组,差异有统计学意义(t=23.04,P<0.01;t=11.38,P<0.01);观察组患者中Ⅲ~Ⅳ期患者的CD4+CD25+Foxp3+Treg的绝对数量和所占CD4+T淋巴细胞比例均显著高于Ⅰ~Ⅱ期患者,差异有统计学意义(t=17.37,P<0.01;t=18.72,P<0.01)。结论在EM的发生、发展中CD4+CD25+Foxp3+Treg的免疫负性调节作用扮演重要角色。EM形成过程中CD4+CD25+Foxp3+Treg数量的增加可能是一个重要的因素,其表达水平对EM患者预后、病程和病情的判定在临床上有一定的价值意义。Objective To detect the proportion of CD4＋ CD25＋ Foxp3＋ Treg in peripheral blood mononuclear cells of patients with endometriosis by flow cytometry, explore the relationship between endometriosis and Treg. Methods The clinical data of patients with endo- metriosis treated in the hospital from August 2012 to April 2014 （observation group） were analyzed; the healthy female volunteers without endometriosis during no-pregnancy and no-menstrual period were selected as control group. The absolute numbers of CD4＋CD25＋Foxp3＋ Treg and the percents in CD4＋Treg in patients of different stages in observation group and control group were compared. Results A total of 78 patients were included in the study, including 45 patients in observation group and 33 patients in control group. The absolute number of CD4＋CD25＋Foxp3＋Treg and the percent in CD4＋ Treg in observation group were statistically significantly higher than those in control group （ t = 23.04, P〈0. 01 ; t = 11.38, P〈0. 01 ） ; in observation group, the absolute number of CD4＋ CD25 ＋ Foxp3 ＋Treg and the percent in CIM＋ Treg in patients of stage Ⅲ - Ⅳ were statistically significantly higher than those in patients of stage Ⅰ-Ⅱ （ t = 17.37, P〈0. 01 ; t = 18. 721 P〈0. 01 ） . Conclusion The negative immunoregulation effect of CIM＋ CD25 ＋ Foxp3 ＋ Treg plays an important role in occurrence and devel- opment of endometriosis; in the pathogenesis of endometriosis, the increase of CD4＋ CD25 ＋ Foxp3 ＋Treg number may be an important factor, the expression level has a certain value and significance in prediction of prognosis, course of the disease, and the patients＇ condition.

关 键 词：子宫内膜异位症 CD4+ CD25+ Foxp3+ TREG 相关性 

分 类 号：R711.71[医药卫生—妇产科学]