血清IgG抗体含量与口腔厌氧菌致牙髓感染的关系  

Relationship between contents of serum IgG antibody and pulp infections caused by oral anaerobic bacteria

作　　者：吕朋君 马珅 刘晓斌[1] 

机构地区：[1]天津市口腔医院牙体牙髓一科,天津300041

出　　处：《中华医院感染学杂志》2015年第16期3780-3782,共3页Chinese Journal of Nosocomiology

基　　金：天津市科技局研究基金资助项目(1203684)

摘　　要：目的利用抗原与抗体特异反应的特点,通过检测血清抗体IgG水平分析口腔厌氧菌与牙髓感染的关系,以降低感染率。方法将2013年1月-2014年1月患有不同牙髓疾病的患者分为B、C、D组,每组各10例,另选10名健康受试者为A组;应用ELISA方法测定健康受试组与感染组患者的血清中10株国际口腔厌氧标准菌株的IgG抗体含量,特异性分析牙髓感染与口腔厌氧菌的关系,数据采用SPSS 17.0软件进行统计分析。结果健康受试组的中间普氏菌血清抗体平均值显著低于B、C、D组(P<0.05);且各组OD值与A组健康受试者相比>2.1,全部为阳性;健康受试组的牙龈卟啉单胞菌血清抗体平均值与牙髓感染组、牙髓-牙周联合病症组患者差异有统计学意义(P<0.05),但与牙周疾病组患者相比较,差异无统计学意义;各组厌氧菌血清抗体OD值之间差异均无统计学意义。结论通过ELISA方法分析与牙髓感染相关的厌氧菌,此种方法无需微生物培养,且其检出率及精确度远高于传统的微生物培养法,可作为临床上检测牙髓感染致病菌的常规方法。OBJECTIVE To observe the relationship between the level of serum IgG antibody and the pulp infections caused by oral anaerobic bacteria by referring to the characteristics of specific response between antigen and anti‐body so as to reduce the infection rate .METHODS The patients with pulp diseases who were treated in the hospi‐tal from Jan 2013 to Jan 2014 were enrolled in the study and divided into the group B ,C ,and D ,with 10 cases in each ;meanwhile 10 healthy subjects were chosen as the group A .The IgG antibody contents in the 10 internation‐al standard anaerobic bacteria strains isolated from the serum of the patients with infections and the healthy sub‐jects were determined by using ELISA method ,the relationship between the pulp infections and the oral anaerobic bacteria was specifically analyzed ,and the statistical analysis of data was performed with the use of SPSS 17 .0 software .RESULTS The average level of serum antibody in Prevotella intermedia was significantly lower in the group A than in the group B ,C ,and D （P〈0 .05） .As compared with the group A ,the OD values of other three groups were more than 2 .1 ,and all were positive .There was significant difference in the average level of serum antibody in Porphyromonas gingivalis among the healthy subjects ,the patients with pulp infections ,and the pa‐tients with pulp‐periodontal diseases （P〈0 .05） ,as compared with the patients with periodontal disease ,howev‐er ,the difference was not significant .There was no significant difference in the OD value of serum antibody in the anaerobic bacteria among the four groups .CONCLUSION The ELISA ,as is applied for the analysis of the anaero‐bic bacteria causing the pulp infections ,may contribute to considerably higher isolation rate and accuracy than the traditional microbial culture ,and it can be used as a conventional method for the detection of pathogenic bacteria causing pulp infections .

关 键 词：IGG抗体 ELISA方法 牙髓感染 口腔 厌氧菌 

分 类 号：R181.32[医药卫生—流行病学]