SIRT1在有氧运动改善APOE^(-/-)小鼠肝脏脂代谢异常中的作用研究  

Research on Effect of SIRT1 on Ameliorating Hepatic Lipid Metabolism Disorder of APOE^(-/-) after Aerobic Exercise

作　　者：燕小妮[1] 

机构地区：[1]怀化学院体育系,湖南怀化418000

出　　处：《体育科学》2015年第8期40-44,共5页China Sport Science

摘　　要：目的:探讨SIRT1在有氧运动改善APOE-/-小鼠肝脏脂代谢异常中的作用。方法:16只雄性C57BL/6J小鼠随机分为C57BL/6J小鼠对照组(CC)和运动组(CE),16只雄性APOE-/-小鼠随机分为APOE-/-小鼠对照组(AC)和运动组(AE),每组8只。12周有氧运动干预后,通过病理学切片观察肝脏脂质积聚情况,检测小鼠肝脏SIRT1、LKB1、P-LKB1-ser428、AMPKα1、P-AMPKα1-Thr172和FAS蛋白表达量。结果:1)与CC组比较,AC组肝脏脂肪积聚增加,与AC组比较,AE组肝脏脂质积聚减少;2)与CC组比较,CE组SIRT1(P<0.05)、P-LKB1-ser428(P<0.01)和P-AMPKα1-Thr172(P<0.01)含量明显升高,LKB1、AMPKα1和FAS没有明显变化(P>0.05);3)与CC组比较,AC组SIRT1(P<0.05)、PLKB1-ser428(P<0.01)和P-AMPKα1-Thr172(P<0.01)表达明显降低,LKB1、AMPKα1和FAS没有明显变化(P>0.05);4)与AC组比较,AE组SIRT1(P<0.01)、P-LKB1-ser428(P<0.01)和P-AMPKα1-Thr172(P<0.01)表达明显升高,FAS表达明显降低(P<0.01),LKB1和AMPKα1没有明显变化(P>0.05)。结论:12周有氧运动可能通过增加SIRT1表达,提高LKB1和AMPKα1活性,抑制FAS表达,改善APOE-/-小鼠肝脏脂代谢异常。Objective ：To observe the effect of SIRT1 of aerobic exercise on the improvement liver lipid metabolism in APOE^‐/‐ rats .Methods ：16 male C57BL/6J rats were randomly divid‐ed into control group （CC ,n= 8） ,exercise group （CE ,n= 8） ,16 male APOE^‐/‐ rats were ran‐domly divided into control group （AC ,n=8） ,exercise group （AE ,n=8） .After 12 weeks in‐tervention ,hepatic lipid accumulation were observed ,Also the protein expression of SIRT1 , LKB1 ,P‐LKB1‐ser428 ,AMPKα1 ,P‐AMPKα1‐Thr172 and FAS were determined in the liv‐er .Results ：1） Compared with CC group ,hepatic lipid accumulation increased significantly in the AC group ,Compared with AC ,lipid accumulation decreased significantly in the AE group . 2） Compared with CC group ,the content of SIRT1 （P〈0 .05） ,P‐LKB1‐ser428（P〈 0 .01） and P‐AMPKα1‐Thr172（P〈 0 .01）increased significantly ,while the expression of LKB1 , AMPKα1 and FAS （P〉 0 .05）didn’t change significantly in the CE group .3） Compared with CC group ,the expression of SIRT 1 （ P 〈 0 .05 ） ,P‐LKB1‐ser‐428 （ P 〈 0 .01 ） and P‐AMPKα1‐Thr172 （ P 〈 0 .01 ） decreased significantly ,while the expression of LKB1 , AMPKα1and FAS（P〉 0 .05）didn’t change significantly in the liver of AC group .4）Com‐pared with AC group ,the content of SIRT1 （P〈 0 .01） ,P‐LKB1‐ser428（P〈 0 .01）and P‐AMPKα1‐Thr172（P〈0 .01）increased significantly ,while that of FAS（P〈 0 .01）decreased significantly ,and that of LKB1 and AM PKα1 didn’ t change obviously （ P〉 0 .05 ） .Conclu‐sions ：12 weeks aerobic exercise may be through promoting the SIRT1 ,increasing LKB1 and AM PKα1 activity ,inhibiting the expression of FAS ,to ameliorate hepatic lipid metabolism dis‐order of APOE^‐/‐ rats .

关 键 词：有氧运动 肝脏脂代谢 动脉粥样硬化 动物实验 鼠 

分 类 号：G804.7[文化科学—运动人体科学]