藏鸡和科宝肉鸡鸡胚卵黄膜、尿囊膜HIF-HIF-2α和VEGF基因表达的比较分析  

Comparison Analysis on HIF-2 Alpha and VEGF Gene Expression in Embryonic Yolk-sac Membrane and Chorioallantoic Membrane between Tibetan and Cobb Broiler Chickens

作　　者：胡巧[1] 董传豪 罗荣翠 安玲丽 徐亚欧[1] 妥丽荣 冯卫东[2] 饶开晴[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]甘孜州畜牧业科学研究所,四川康定626000

出　　处：《中国家禽》2015年第15期11-14,共4页China Poultry

基　　金：西南民族大学生科院本科生创新型科研项目;西南民族大学研究生创新型科研项目(CX2015SZ082);四川省科技项目应用基础项目(2013JY0044)

摘　　要：藏鸡和肉鸡在耐低氧性状上有明显的差异,但对品种间这种差异的机制研究还未见报道。试验通过RT-PCR对高原低氧孵化的藏鸡和常氧孵化的肉鸡(科宝500)14日胚龄鸡胚卵黄膜、尿囊膜(CAM)HIF-2α和VEGF m RNA表达量进行检测和比较,从分子水平上分析不同品种之间的表达差异。结果发现藏鸡鸡胚卵黄膜和CAM组织中HIF-2αm RNA表达量均显著高于肉鸡鸡胚卵黄膜、CAM内表达(P<0.05)。藏鸡鸡胚CAM组织中VEGF m RNA表达量显著高于肉鸡CAM内表达(P<0.05),而卵黄膜组织中VEGF m RNA表达没有显著差异。藏鸡与肉鸡卵黄膜、CAM组织中HIF-2α和VEGF基因表达量均不相关。结果提示,HIF-2α基因在藏鸡鸡胚卵黄膜、CAM均高度表达,可能是藏鸡对低氧适应的结果;而VEGF基因在藏鸡CAM高度表达,这可能更有利于藏鸡鸡胚CAM血管的形成,进而提高藏鸡在高原低氧环境中的适应能力。藏鸡与肉鸡鸡胚卵黄膜、CAM组织HIF-2α和VEGF基因可能通过不同的途径发挥作用。There are significant differences in the resistance to hypoxia traits between Tibetan and broiler chicken（Cobb 500）, but the mechanism of differences between breeds has not been reported. In this experiment, reverse transcription PCR was used to detect and compare the expression levels of HIF-2α,VEGF mRNA in yolk-sac membrane and chorioallantoic membrane （CAM） on embryonic day 14 （E14） of Tibetan chicken hatched at high altitude hypoxia and broiler chicken hatched at normoxic environment. The expression difference between the two breeds were analysed at the molecular level. The results showed that HIF-2α mRNA expression level in yolk-sac membrane and CAM of Tibetan chicken was significantly higher than those of broiler chicken on E14 （P〈0.05）. Expression of VEGF mRNA in CAM was higher than that of broiler chicken （P〈0.05）,but there was no significant difference in yolk-sac membrane between the two breeds （P〉0.05）. There was no correlation between HIF-2α and VEGF mRNA expression in yolk-sac membrane and CAM tissue between Tibetan and broiler chicken. The results suggested that HIF-2α gene was highly expressed in yolk-sac membrane,CAM of Tibetan chicken, and it may be the result of hypoxic acclimatization of Tibetan chicken. While VEGF gene was highly expressed in CAM of Tibetan chicken,which may be more beneficial to vessel angiogenesis in order to improve the adaptability of Tibetan chicken embryos in high altitude hypoxia environment. HIF-2α and VEGF genes of Tibetan and broiler chicken in embryonic yolk-sac membrane,CAM might play a role via different ways.

关 键 词：藏鸡 肉鸡 卵黄膜 CAM HIF-2α VEGF 基因表达 

分 类 号：S831.1[农业科学—畜牧学]