亳芍组织培养研究  

Study on the Tissue Culture Technique of Bozhou Peony in vitro Culture

作　　者：李文峰 马宗新 马同富[2] 

机构地区：[1]阜阳市农业科学院,安徽阜阳236065 [2]阜阳师范学院,安徽阜阳236041

出　　处：《安徽农业科学》2015年第25期24-25,91,共3页Journal of Anhui Agricultural Sciences

基　　金：安徽省教育厅高校自然科学基金重点项目"芍药;牡丹药用植物种质资源的收集;研究及利用"(KJ2013A207)

摘　　要：[目的]研究亳州芍药组培、脱毒快繁关键技术。[方法]以亳州芍药地下嫩芽为外植体,研究不同取材时间、材料的放置时间和培养基激素配比对芍药组织培养的影响。[结果]亳州芍药地下嫩芽的取材应以2月份为宜,选取个体中等、尚未完全展开的地下嫩芽。在相同材料的前提下,放置时间越短的植物材料成活率越高,但其褐化率也高。对于不同放置时间的地下嫩芽,要综合考虑污染率和褐化率,选择不同的处理。愈伤组织在接种20 d左右时生长很旺盛,30 d前后芽体生长比较旺盛,这一时期有利于扩大培养。扩大培养的时间应选择在20-30 d。[结论]该研究为其幼苗提供质量保障,并为农民获得安全放心苗提供技术依托。[ Objective ] The aim was to study tissue culture technique of Bozhou Peony in vitro culture. [ Method ] Bozhou Peony cultured in vitro was studied for effect of sampling time, storage period, combination of plant growth regulators. [ Result ] The results showed that the unfolding medium underground buds in February were appropriate. The short time of the material, the inoculation survival rate was high, but the brown rate was also high. Therefore, different storage time of underground buds should choose different treatment. Callus growth was very strong in the twentieth day after inoculation while bud growth was strong in the thirtieth day, expand training was very favorable in the period. Expand culture time should be selected between 20 and 30 days. [ Conclusion ] The study provided quality assurance for the seedlings, and provided technology support for safe seedlings.

关 键 词：亳州芍药 组织培养 愈伤组织 

分 类 号：S604.3[农业科学—园艺学] Q813.12[生物学—生物工程]