新城疫病毒的分离与鉴定  

Isolation and Identification of Newcastle Disease Virus

作　　者：徐丹雯 魏和朋 胡顺林[1] 石火英[1] 

机构地区：[1]扬州大学兽医学院病理教研室禽类预防医学教育部重点实验室,江苏扬州225009

出　　处：《安徽农业科学》2015年第25期117-119,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(31172300);江苏省高校自然科学重大项目(12KJA230002);高等学校博士学科点专项科研基金(博导类)项目(20133250110002);江苏高校优势学科建设工程资助项目(PAPD);江苏省动物重要疫病与人兽共患病防控协同创新中心项目

摘　　要：[目的]对活禽市场进行新城疫病毒的流行病学调查。[方法]从活禽市场采集40份鸡泄殖腔棉拭子,由SPF鸡胚扩增病毒,收集病毒尿囊液;采用RT-PCR技术扩增新城疫病毒的DNA,并测序。[结果]共分离出4株新城疫病毒。通过对分离株F蛋白的氨基酸序列分析发现,分离株裂解位点附近的氨基酸残基组成均为112ERQERL117,符合新城疫病毒弱毒株裂解序列特征,而且分离到的NDV之间具有较高的同源性,介于86.3%-93.9%,并与疫苗株La Sota的F基因核苷酸序列属于同一分支。[结论]小型活禽市场中鸡携带了弱毒新城疫病毒,因此应加强活禽市场的管理。[ Objective ] The research aimed to make the epidemiological investigation on the newcastle disease in live poultry market. [ Metbod ] 40 cloacal swabs were collected from several live poultry markets. The virus in SPF eggs was amplified to collect the allantoic fluid of virus. DNA of NDV was amplified by using RT-PCR and sequenced. [ Result] Four strains of new newcastle disease virus were isolated. The amino acid sequence analysis of F protein of the isolated strains showed that the amino acid residues at near cleavage sites of the isolated strain were 112ERQERL117, which displayed according with the characteristics of attenuated newcastle disease virus. F gene of the isolated strains had the homology of 86.3% -93.9%, and they were classified into the same branch with the vaccine strain LaSota. [ Conclusion] The chicken carried the low-virulence newcastle disease virus in the small live poultry market, so the management of the live poultry market should be strengthened.

关 键 词：新城疫病毒 活禽市场 分离 鉴定 序列分析 

分 类 号：S858.31[农业科学—临床兽医学]