糖肾安对早期糖尿病大鼠耳廓及肾脏微循环血流量的影响  

The Effect of Tang Shenan Decoction on Microcirculation Blood Flow in Auricle and Kidney of Early Diabetic Rats

作　　者：石玉婷[1] 周陈陈 王镁[2] 于世家[2] 

机构地区：[1]辽宁中医药大学研究生学院,沈阳110847 [2]辽宁中医药大学附属医院内分泌科,沈阳110032

出　　处：《微循环学杂志》2015年第3期5-8,F0002,共5页Chinese Journal of Microcirculation

基　　金：沈阳市科技局项目(F14-231-1-11)

摘　　要：目的:应用激光散斑成像(LSI)技术观察中药复方糖肾安煎剂对早期糖尿病(DM)大鼠耳廓及肾脏血流量的影响。方法:30只SD大鼠,采用高糖高脂饮食喂饲4周并一次性腹腔注射小剂量链脲佐菌素(STZ,35mg/kg)建立DM大鼠模型,将成模的28只大鼠随机分为DM模型组(n=9)、西药治疗组(胰激肽原酶肠溶片治疗,n=9)和中药治疗组(糖肾安煎剂治疗,n=10),另选10只SD大鼠作为正常对照组。药物治疗8周后,用moor FLPI-2全景激光灌注成像仪监测各组大鼠耳廓及肾脏微循环血流量变化。结果:DM模型组大鼠耳廓及肾脏血流量为133.42±49.58LSPU和297.19±39.43LSPU,正常对照组为70.04±7.28LSPU和239.27±33.27LSPU;中药治疗组为77.11±14.16LSPU和253.6±35.57LSPU,西药治疗组为82.71±14.03LSPU和248.96±39.26LSPU。DM模型组上述检测值均高于正常对照组(P<0.01);中药治疗组和西药治疗组上述检测值均显著低于DM模型组(P<0.01);中药治疗组、西药治疗组和正常对照组组间血流量差异无统计学意义(P>0.05)。结论:糖肾安煎剂能明显减轻早期DM大鼠微循环障碍引起的微血管血流量增加,其效果与胰激肽原酶相当。Objective： To observe the effect of tang shenan decoction on microcirculation blood flow in early diabetic rat auricle and kidney by using laser speckle imaging （LSI） technology. Method： 30 SD rats were choosen to establish the diabetic rats models by high-lipids and high-sucrose diets for 4th week and then intraperitoneal injection of one-time （STZ, 35mg/kg） , and then they were randomly divided into DM model group（n=9）, western medicine- treated group （n= 9）and chinese medicine-treated group（n= 10）, another 10 SD rats were served as the control group. All groups were given relevant medicinal intragastrically. The changes of rats auricle and kidney microcirculation blood flow were detected by a Moor-full field laster perfusion imager （Moor-FLPI） at the end of 8th week. Results： The rats aurical and kidney blood flow of DM model were 133.42±49.58LSPU and 297.19.85±39.43LSPU. The control group were 70.04±7.28LSPU and 239.27±33.27LSPU. Both chinese medicine-treated group 77.11±14. 16LSPU,253.6±35.57 LSPU and western medicine-treated group 82.71±14.03LSPU,248.96±39.26 LSPU were obviously lower than the DM model group, and the differences were significant（P〈0.01） ,but we found no statistical difference among chinese medicine-treated group, western medicine-treated group and the control group （P〈0.05）. Conclusion; Tang shenan decoction have the equivalent efficacy with pancreatic kininogenase in reducing the blood flow of rats aurical and kidney which was caused by early-stage diabetic.

关 键 词：糖肾安煎剂 胰激肽原酶肠溶片 激光散斑成像技术 微循环血流量 糖尿病大鼠 

分 类 号：R587.2[医药卫生—内分泌]