促甲状腺激素受体可在微血管内皮细胞表达  

Expression of Thyroid Stimulating Hormone Receptor on Microvascular Endothelial Cells

作　　者：杨光燃[1] 杨芳远 曹曦[1] 杨金奎[1] 

机构地区：[1]首都医科大学附属北京同仁医院内分泌科,北京100730

出　　处：《微循环学杂志》2015年第3期9-12,共4页Chinese Journal of Microcirculation

基　　金：国家自然科学基金项目(81471009);北京市卫生系统高层次卫生技术人才培养计划(2014-3-013);首都医科大学基础临床科研合作课题(14JL46)

摘　　要：目的:检测促甲状腺激素受体(TSHR)在小鼠脑微血管内皮细胞(bEnd.3)的表达。方法:取对数生长期bEnd.3细胞,采用RT-PCR和Western Boltting方法检测bEnd.3细胞TSHR mRNA和蛋白表达水平。结果:bEnd.3细胞总RNA浓度为2.9186μg/μl,A260/A280为1.97。TSHR mRNA的扩增效率为96.41%±0.82%,溶解曲线呈单峰,相对表达量为0.64±0.14(相对睾丸组织)。电泳结果显示bEnd.3细胞能清晰表达TSHR mRNA。Western Blotting显示bEnd.3细胞可以检测到TSHR的α和β亚基蛋白表达,β亚基相对表达量为0.46±0.05(相对β-actin)。结论:小鼠脑微血管内皮细胞可表达TSHR,该结果有助于临床研究TSH对血管内皮细胞功能的影响。Objective： To evaluate the expression of thyroid stimulating hormone receptor （TSHR） on mouse microvascular endothelial cells （bEnd. 3）. Method： Real-time PCR and Western-boltting were used to analyze the expression of TSHR mRNA and TSHR protein in the bend. 3 cells. Results： TSHR mRNA expression was detected in the bend. 3 cells. The content of RNA in the bend. 3 cells was 2. 9186μg/μl, and its ratio of A260/A280 was 1. 97. TSHR was amplified and the amplification efficiency was 96.41% ±0.82%. Dissociation curve from real-time PCR was unimodal. The relative quantity of TSHR mRNA in the bEnd. 3 cells was 0.64±0.14, when the quantity of TSHR mRNA in the testicle was set as 1.0. TSHR a and i3 subunit were detected by Western-blotting. The protein ratio of TSHRβ/β-actin was 0.46±0.05 in the gray scale analysis. Conelusion.. The expression of TSHR could be found in the bend. 3 cells. This finding may be helpful to explore the effect of thyroid stimulating hormone on microvascular endothelial cells.

关 键 词：促甲状腺激素受体 微血管 内皮细胞 

分 类 号：R581[医药卫生—内分泌]