紫杉醇己内酯/碳酸亚乙酯载药纳米涂层制备及其体外实验研究  

Establishment of paclitaxel loaded ε-aprolactone/ethylene carbonate nano coating and its in vitro experiment

作　　者：杨进[1] 梅劲桦[1] 刘震杰[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院血管外科,浙江杭州310016

出　　处：《中国医药科学》2015年第1期78-81,共4页China Medicine And Pharmacy

基　　金：浙江省卫生厅科研项目资助(2012KYA110)

摘　　要：目的构建紫杉醇己内酯(CL)/碳酸亚乙酯(EC)载药纳米涂层,考察其体外释放情况并评价其对血管内皮细胞和平滑肌细胞存活率的影响。方法结合异步降解技术和静电纺织技术制备紫杉醇Poly(CLco-EC)载药纳米涂层;高压液相色谱法测定载药体系紫杉醇包封率和体外释放曲线;MTT实验测定人血管内皮细胞和平滑肌细胞存活率。结果 CL/EC=6和CL/EC=9的紫杉醇Poly(CL-co-EC)的药物包封率分别为92.1%和79.2%;载药量分别为26.5%和18.1%;紫杉醇在体外缓慢释放,50d后累计释放量分别达到78%和37%。紫杉醇Poly(CL-co-EC)电纺薄膜显著降低血管内皮细胞和平滑肌细胞的存活率。结论紫杉醇Poly(CL-co-EC)载药纳米涂层具有缓慢释放和释放速率可控的特点,具有作为血管内支架的潜在价值。Objective To establish a nano-coating of paclitaxel loaded ε-aprolactone/Ethylene carbonate copolymer and investigate the release of the nano coating and effects on inhibition ratio of vascular endothelial and smooth muscle cells. Methods The technology of asynchronous degradation and electrostatic spinning technique were used to prepare nano-drug carrier. High pressure liquid chromatography （HPLC） was used to determine the embedding ratio and release curve of paclitaxel loaded nano coating. MTT was applied to test cells survival rate. Results The embedding ratios of paclitaxel in nano-coatings of CL/EC=6 and CL/EC=9 were 92.1%and 79.2%, and drug-loading rate were 26.5% and 18.1%, respectively. Paclitaxel in nano-coatings were slow-release and the accumulative release quantities were 78% and 37% in vitro. The nano-coatings significantly reduced the survival rate of vascular endothelial and smooth muscle cells. Conclusion The nano-coatings of paclitaxel loaded ε-aprolactone/ethylene carbonate copolymer established in this study slowly releases paclitaxel and inhibits the growth of vascular endothelial and smooth muscle cells, which supplies a potential value in stent implantation.

关 键 词：己内酯 碳酸亚乙酯 紫杉醇 纳米涂层 血管支架 

分 类 号：R318.08[医药卫生—生物医学工程]