2例1型糖尿病患者外周血TCR Vβ7分子结构模拟与分析  

Analysis and simulation of TCR Vβ7 molecular structure of peripheral blood from two patients with type 1 diabetes

作　　者：周建伟[1] 贾印峰[1] 王丽[2] 孔翠[3] 金呈强[1] 董海新[1] 

机构地区：[1]济宁医学院附属医院检验科,山东济宁272029 [2]山东省济宁市第一人民医院药学部,山东济宁272011 [3]济宁医学院附属医院护理部,山东济宁272029

出　　处：《中国医药科学》2015年第7期47-49,共3页China Medicine And Pharmacy

基　　金：山东省自然科学基金(ZR2012HL29);山东省高等学校科技计划项目(J11LF18);山东省济宁市科技发展计划项目([2011]57)

摘　　要：目的模拟和分析2例1型糖尿病(T1D)患者外周血TCR Vβ7的结构。方法以荧光定量PCR熔解曲线分析技术检测T1D患者外周血TCR Vβ7的谱系漂移,利用IMGT数据库和生物信息学软件(CPH Models 2.0 Server和Ras Mol 2 Software)模拟分析其分子结构。结果 2例T1D患者外周血的TCR Vβ7拥有极为相似的氨基酸序列"CASRTAGQYEQYFGPGTR"和"CASRTAGQYEQFFGPGTR",唯一不同的是第十二个氨基酸残基,前者为酪氨酸(Y),后者为苯丙氨酸(F)。分子模拟显示,两者TCR Vβ7基因结构明显不同。结论不同T1D患者来源的TCR Vβ7即使具有相似或相同的氨基酸序列,分子结构未必相同;其可能来自于不同的T细胞克隆。Objective To compare the sequences and crystal structures of TCR Vβ7 obtained from two patients with type 1 diabetes（T1D）.Methods The skewness of TCR Vβ7 of two T1D patients were detected with real-time florescence quantitative polymerase chain reaction （RQ-PCR） and DNA melting curve analysis technique followed by being sequenced, the crystal structures of them were simulated according to IMGT database, CPH Models2.0 Server and RasMol 2 Software.Results The whole sequences of TCR Vβ7 of T1D patient-1 were “CASRTAGQYEQYFGPGTR”, that of patient-2 were “CASRTAGQYEQFFGPGTR”, the only difference between them lied on the twelfth amino acid. The crystal structures of Vβ7 of the two patients simulated with stick model were rather similar, while that with sphere model were obviously different.Conclusion Although sharing the similar or same gene sequences, the crystal structures of the TCR Vβ7 obtained from different T1D patients probably are different.

关 键 词：1型糖尿病 外周血 T细胞抗原受体 结构模拟 

分 类 号：R392.6[医药卫生—免疫学]