围绝经期妇女PD-1的表达及意义  

The expression and significance of PD-1 inperimenopausal and postmenopausal women

作　　者：吴静[1] 王琳[2] 凌晨[2] 祁松楠 赵瑞珂 顾国浩[2] 

机构地区：[1]江苏省盐城市妇幼保健院检验科,江苏盐城224002 [2]苏州大学附属第一医院检验科,江苏苏州215006

出　　处：《中国医药科学》2015年第9期13-16,57,共5页China Medicine And Pharmacy

摘　　要：目的探讨围绝经期妇女外周血单个核细胞共刺激分子PD-1m RNA和T细胞表面PD-1分子的表达和意义。方法采用Taqman探针实时荧光定量PCR法、流式细胞术检测70例围绝经期、40例绝经后期和30例育龄期组外周血单个核细胞PD-1m RNA的表达。结果 PD-1m RNA的表达在三组间比较差异有统计学意义(P<0.05),与育龄期组相比,围绝经期组及绝经后期组PD-1m RNA表达量均显著增高,差异有统计学意义(P<0.05);T细胞表面PD-1的表达在三组间比较差异有统计学意义(P<0.05),围绝经期及绝经后期组CD4+T细胞和CD8+T细胞的PD-1水平低于育龄期组(P<0.05)。结论共刺激分子PD-1的异常表达可能与围绝经期妇女免疫功能失调有关。Objective To investigate the expression of PD-1 mRNA in peripheral blood mononuclear cells and the expression of PD-1 on T cells in perimenopausal and postmenopausal women. Methods The mRNA expression of PD-1 mRNA were detected by Taqman probe real-time quantitative PCR,and the levels of PD-1 on T cells were determined by Flow Cytometry.The subjects in the study included 70 perimenopausal women,40 postmenopausal women and 30 childbearing age women. Results The expression ofPD-1 mRNA differed significantly in these three group（P 〈 0.05）,compared with the childbearing age group,PD-1 mRNA level in postmenopausal women and postmenopausal women was significantly increased（P 〈 0.05）;The expression ofPD-1 on T cells differed significantly in these three group（P 〈 0.05）,compared with the childbearing age group,PD-1 level in postmenopausal women and postmenopausal women was significantly decreased（P 〈 0.05）. Conclusion The abnormal expression of PD-1 may be closely related to the immune dysfunction of perimenopausal women.

关 键 词：围绝经期 实时荧光定量PCR 流式细胞术 免疫功能 PD-1 

分 类 号：R711.51[医药卫生—妇产科学]