HPLC同时测定益脑胶囊中五味子醇甲和五味子乙素的含量  

Determination of schisandrin and γ-Schizandrin in Yinao capsules by HPLC

作　　者：刘晓明 雷蓉[1,2] 刘永利 王璐 

机构地区：[1]河北省药品检验研究院,河北石家庄050011 [2]河北医科大学药学院,河北石家庄050011

出　　处：《中国医药科学》2015年第9期52-54,共3页China Medicine And Pharmacy

摘　　要：目的：建立使用高效液相色谱法同时测定益脑胶囊中五味子醇甲、五味子乙素的含量，完善、提高益脑胶囊的质量标准。方法 Dikma RP-C18（250mm×4.6mm，5μm）色谱柱；流动相：乙腈（A）-0.1%磷酸溶液（B）；检测波长：250nm；体积流量：1.0mL/min；柱温：30℃。结果该方法的五味子醇甲线性范围在0.01577～1.5768μg（r=0.9999），平均回收率为103.1%，RSD 为1.0%。五味子乙素线性范围在0.004160～0.2080μg（r=0.9999），平均回收率为101.7%，RSD 为1.2%。结论该方法可行、重复性好，能有效控制益脑胶囊中五味子的质量。Objective To establish a HPLC method for the determination of schisandrin,γ-Schizandrin in Yinao Capsule. Methods The determination was performed by HPLC on Dikma RP-C18（250mm×4.6mm,5μm） column. The mobile phase consisted of acetonitrile （A）-0.1% phosphoric acid solution （B） with gradient elution at a flow rate of 1.0mL/min.The detection wavelength was 250nm. Results The linear range of schisandrin was 0.01577-1.5768μg, r=0.9999.The average recovery was 103.1%,RSD=1.0%.The linear range of γ-Schizandrin was 0.004160-0.2080μg, r=0.9999.The average recovery was 101.7%,RSD=1.2%. Conclusion The method is convenient,sensitive and accurate.It can be a method for quality control in production of Yinao capsule.

关 键 词：益脑胶囊 五味子醇甲 五味子乙素 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]