鱼藤属植物内生真菌的分离及杀虫活性研究  

Isolation and insecticidal activity of endophytic fungi in Derris

作　　者：孙涛[1] 孙之潭[1] 胡美英[1] 钟国华[1] 

机构地区：[1]华南农业大学昆虫毒理研究室,广州510642

出　　处：《环境昆虫学报》2015年第4期785-789,共5页Journal of Environmental Entomology

基　　金：广东省自然科学基金(05006634)

摘　　要：本文是以毛鱼藤、白花鱼藤、蜜花鱼藤3种鱼藤属植物为试验材料,分离筛选代谢物具有生物活性的内生真菌菌株。通过组织分离法,从3种鱼藤属植物中分离得到4株代谢表现旺盛的内生真菌菌株。杀虫活性实验表明:4种内生真菌的代谢产物对松材线虫都具有一定的毒杀作用,其中青霉菌的代谢产物活性最高,48 h校正死亡率达90%以上。4种内生真菌中,只有青霉菌的代谢产物对家蚕4龄幼虫具有触杀活性,1.5 mg/头剂量处理时,24 h校正死亡率即达到100%。Derris elliptica Benth. , Derris albo - rubra Hemsl. and Derris thyrsiflora Benth. were taken as experimental material to isolate endophytic fungi which had bioactive secondary metabolites. 4 strains of metabolic vigorously endophytic fungi were obtained and their insecticidal activity were investigated. The result indicated that the secondary metabolites of 4 Bursaphelenchus xylophilus than others, the corrected strains of endophytic fungi in Derris had toxic effect on N. The metabolites of Penicillium sp. showed the more significant activity mortality rate reaching over 90% within 48 hours. Moreover, only the metabolites of Penicillium sp. showed larvicidal efficiency against 4th instar larvae of Bombyx mori L. the corrected mortality rate reaching 100% at the dosage of 1.5 mg/larva.

关 键 词：鱼藤 内生真菌 代谢产物 家蚕 松材线虫 

分 类 号：Q965.9[生物学—昆虫学] S476.12[农业科学—农业昆虫与害虫防治]