HPLC法同时测定柴银口服液中5种成分的含量  

Simultaneous determination of five components in Chaiyin Oral Liquid by HPLC

作　　者：朱日然[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250011

出　　处：《药学研究》2015年第9期509-511,共3页Journal of Pharmaceutical Research

摘　　要：目的建立高效液相色谱法同时测定柴银口服液中5种成分的含量.方法用Zorbaxsb-C18色谱柱（4.6mm×250mm,5μm）分离,乙腈-0.1%磷酸溶液为流动相梯度洗脱,流速为1.0mL·min^-1,检测波长为210nm.结果绿原酸、苦杏仁苷、葛根素、黄芩苷、连翘苷的线性范围分别为0.388～9.70μg（r=0.9999）、0.736～18.40μg（r=0.9998）、0.21～5.25μg（r=0.9998）、0.242～10.6μg（r=0.9999）、0.868～21.7μg（r=0.9999）,平均加样回收率分别为96.80%（RSD=0.29%）、103.50%（RSD=129%）、103.60%（RSD=1.12%）、102.90%（RSD=0.88%）、100.90%（RSD=0.90%）.结论该方法简便、准确,重复性好,可用于柴银口服液的质量控制.Objective To establish an HPLC method for the simultaneous determination of five components in Chaiyin Oral Liquid. Methods Using Zorbax SB- C18column( 4. 6 mm × 250 mm,5 μm),acetonitrile- 0. 1% phosphoric acid solution as mobile phase. The flow rate was 1 m L· min- 1,the detection wavelength was at 210 nm. Results The linear ranges of chlorogenic acid,amygdalin,puerarin,baicalinand,phillyrin were 0. 388 ~ 9. 70 μg( r = 0. 999 9),0. 736 ~ 18. 40μg( r = 0. 999 8),0. 21 ~ 5. 25 μg( r = 0. 999 8),0. 242 ~ 10. 6 μg( r = 0. 999 9),0. 868 ~ 21. 7 μg( r = 0. 999 9),respectively. The average recoveries( n = 6) were 96. 80%( RSD = 0. 29%),103. 50%( RSD = 1. 29%),103. 60%( RSD =1. 12%),102. 90%( RSD = 0. 88%),100. 90%( RSD = 0. 90%),respectively. Conclusion The results indicated that the method was simple,accurate,highly selective and reproducible,thus it can be used as quality control for Chaiyin Oral Liquid.

关 键 词：柴银口服液 绿原酸 苦杏仁苷 葛根素 黄芩苷 连翘苷 

分 类 号：R927.2[医药卫生—药学]