多倍体中华鲟微卫星亲子鉴定体系的建立  

Parentage identification of polyploidy Acipenser sinensis based on microsatellite markers

作　　者：辛苗苗[1,2] 张书环[2] 汪登强[2] 汪珂[2] 张磊[2] 霍来江 危起伟[2,3] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]农业部淡水生物多样性保护重点实验室,中国水产科学研究院长江水产研究所,武汉430223 [3]中国水产科学研究院淡水渔业研究中心,江苏无锡214081

出　　处：《淡水渔业》2015年第4期3-9,共7页Freshwater Fisheries

基　　金：公益性行业(农业)科研专项:珍稀水生动物繁育与物种保护技术研究(201203086)

摘　　要：应用荧光引物和自动测序技术，检测了10个自主研发的微卫星分子标记在68个中华鲟（ Acipenser sinensis）个体中的遗传多态性。结果表明，10个微卫星标记共检测到124个等位基因，等位基因数为7～22，期望杂合度为0.73026～0.87395， Shannon－Wiener多样性指数为1.56161～2.21960。基于亲子鉴定排除率不低于0.99的标准，最终确定Asi－75067、 Asi－67648、 Asi－67123、 Asi－73843、 Asi－72040、 Asi－70421和Asi－56700等7个微卫星标记为中华鲟亲子鉴定的核心体系，其单亲、父权和双亲的累积排除率分别为99.12％、99.95％和99.99％。该微卫星标记组合为中华鲟准确高效经济的亲子鉴定体系建立提供了科学依据。10 novel microsatellite markers were used for assessing genetic diversity of 68 Acipenser sinensis individuals.The results demonstrated that there were 124 alleles in 10 loci and the number of alleles, mean expected heterozygosities and Shannon-Wiener Diversity Indices per locus ranged from 7 to 22, from 0.730 26 to 0.873 95 and from 1.561 61 to 2.219 60, respectively.With the criterion that cumulative exclusion probabilities were not lower than 0.99, the 7 high polymor-phic microsatellite markers （Asi-75067, Asi-67648, Asi-67123, Asi-73843, Asi-72040, Asi-70421 and Asi-56700） made up the system of paternity test for A.sinensis.The cumulative exclusion probabilities over the 7 loci of single parent, paternity and pair parent were 0.991 232, 0.999 503 and 0.999 997, respectively.These high polymorphic mic-rosatellite markers provided a scientific basis for validating paternity testing efficiently with a lower cost in A.sinensis.

关 键 词：微卫星 中华鲟( ACIPENSER sinensis) 亲子鉴定 多倍体 累积排除率 

分 类 号：S917.4[农业科学—水产科学]