中国大鲵“烂脚病”病原菌的分离鉴定  

Isolation and identification of pathogenic bacteria caused “foot-rot disease”in Andrias davidianus

作　　者：樊汶樵[1] 孙翰昌[1] 姜玉松[1] 徐敬明[1] 蒋丽琳[1] 赵满春[1] 吴宝红[1] 

机构地区：[1]重庆文理学院林学与生命科学学院重庆珍稀濒危水产资源保护与开发研究中心,重庆402168

出　　处：《淡水渔业》2015年第4期100-103,共4页Freshwater Fisheries

基　　金：重庆市教委科学技术研究项目(KJ1401105);重庆市基础与前沿研究计划一般项目(cstc2014jcyj A80042);重庆市科委自然科学基金计划资助项目(Cstc2011jj A80028);重庆文理学院人才引进项目(R2013LS13;R2014LX07)

摘　　要：为了解重庆地区部分中国大鲵(Andrias davidianus)养殖场流行"烂脚病"的病因,对患"烂脚病"中国大鲵进行剖检后,在其肺部分离到一株优势菌CQWU2013,对该优势菌进行致病性实验、生理生化特性鉴定、16S r DNA测序和药敏实验。结果显示:中国大鲵"烂脚病"是由肺炎克雷伯氏菌(Klebsiella mobilis)引起的,该菌为短链状排列的革兰氏阴性杆菌,其生理生化特性符合肺炎克雷伯氏菌的指标,16S r DNA序列同源性高达99.6%;药敏实验显示该菌对部分青霉素类、β-内酰胺类、氨基糖苷类和糖肽类药物耐药,对供试的20种药物中有7种药物出现明显的耐药现象。In order to understand the pathogen from Andrias davidianus suffered from“foot-rot” disease, pathogenic bacte-ria from pathologic tissues of diseased A.davidianus were examined and isolated.Identifications of the isolated bacteria in-cluded the morphological, physiological and biochemical characteristics.The 16S rDNA gene detection and antibiotic sen-sitivity test were also conducted.The result showed only one predominant bacteria strain was isolated and identified as Klebsiella mobilis, and it had strong pathogenicity to giant salamander and crucian carp; the similarity of16 S rDNA was 99.6%to other K.mobilis.The antibiotic susceptibility test revealed that K.mobilis rain was resistant to 7 agents.Above all, the K.mobilis strain was bacterial pathogen leading to “foot-rot” disease of A.davidianus; some candidate sensitive antibiotics was provided for the clinical treatment of the disease.

关 键 词：中国大鲵(Andrias davidianus) “烂脚病” 分离鉴定 耐药性 

分 类 号：S947.3[农业科学—水产养殖]