铅、镉胁迫对云南樟幼苗叶绿素荧光特性的影响  

Effects of Lead and Cadmium Stress on Chlorophyll Fluorescence Characteristics in Cinnamomum glanduliferum Seedlings

作　　者：唐探 姜永雷[1] 张瑛[1] 程小毛[1] 

机构地区：[1]西南林业大学园林学院,昆明650224

出　　处：《湖北农业科学》2015年第11期2655-2658,共4页Hubei Agricultural Sciences

基　　金："省部级重点学科;省高校重点实验室及校实验室共享平台"资助项目;云南省应用基础面上项目(2010ZC264)

摘　　要：选择云南樟[Cinnamomum glanduliferum(Wall.)Nees]二年生实生幼苗为试验材料,用铅(Pb)、镉(Cd)溶液对其进行胁迫处理,研究不同浓度Pb、Cd胁迫对云南樟幼苗光合色素含量及叶绿素荧光参数的影响。结果表明,在Pb胁迫下,随着Pb2+胁迫浓度的增大,叶绿素a含量逐渐升高,Pb2+对云南樟幼苗胁迫程度不大;在Cd胁迫下,随着Cd2+胁迫浓度的增大,叶绿素a及叶绿素b含量都显著下降,Cd2+胁迫对云南樟幼苗造成了严重伤害。叶绿素荧光分析表明,Pb、Cd胁迫处理下云南樟叶片荧光参数F0显著增加,同时Fv/Fm降低,说明在高浓度Pb、Cd胁迫下植株发生了光抑制,造成了PSII的反应中心失活;高浓度Pb2+、Cd2+处理后q N下降幅度显著大于q P,表明Pb、Cd胁迫使光合电子传递能力减弱,叶片暗反应受阻,光合速率下降。Taking the two-years old seedlings of Cinnamomum glanduliferum （Wall.） Nees as experiment materials,the effects of lead and cadmium stress on chlorophyll fluorescence characteristics in C. glanduliferum seedlings were studied. Results showed that, under the stress of lead, compared with the contrast, the content of chlorophyll a gradually raised with the increase of the treatment concentration and the stress effect was not obvious. Under cadmium stress,with the increase of the treatment concentration, the content of chlorophyll a and chlorophyll b decreased significantly and the C. glanduliferum seedlings was damaged. Chlorophyll fluorescence analysis showed that, under the lead and cadmium treatment,the initial fluorescence （F0） increasedin increased significantly and Fv/Fm decreased at the same time,which explained that under the high Pb2＋ and Cd2＋ treatment photoinhibition occurred so as to the inactivation of PSII reaction center, qN decreased significantly greater than qP after the high concentration of lead and cadmium treatment, which showed that the stress of lead and cadmium weakened the transportation ability of photosynthetic electron, blocked blade dark reaction and decreased photosynthetic rate.

关 键 词：云南樟[Cinnamomum glanduliferum(Wall.)Nees] Pb、Cd胁迫 叶绿素荧光 

分 类 号：Q949.747.5[生物学—植物学] Q945.78