呼吸道分离鲍曼不动杆菌生物被膜形成能力与耐药性关系的研究  

Correlation between biofilm production and multiple drug resistance of Acinetobacter baumannii isolated from respiratory tract

作　　者：刘原[1] 柯蕊[1] 杨妮[1] 赵玉杰[1,2] 和平[1] 冯雁京 张立然[1] 耿燕[3] 

机构地区：[1]西安交通大学第二附属医院呼吸内科 [2]西安交通大学第二附属医院重症医学科 [3]西安交通大学第二附属医院检验科,陕西西安710004

出　　处：《中国实验诊断学》2015年第8期1243-1246,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金(81270061);陕西省科技公关项目(2010K16-01(2))

摘　　要：目的分析呼吸道分离鲍曼不动杆菌生物被膜形成能力与耐药性之间的关系。方法采用K-B法测定鲍曼不动杆菌对14种抗菌药物的敏感性,用改良微量板法检测菌株生物被膜形成时间及能力。结果呼吸道分离的鲍曼不动杆菌耐药形势严峻。53.93%的鲍曼不动杆菌可形成生物被膜,生物被膜形成阴性株对多数抗生素的耐药率高于生物被膜形成阳性株。结论呼吸道分离的鲍曼不动杆菌生物被膜形成能力与耐药性呈负相关。Objective To examine the correlation between antibiotic resistance and biofilm formation ability among Acinetobacter baumannii separated from respiratory tract.Methods The K-B method was applied to test drug sensitivi-ty of all strains.The crystal-violet staining method was applied to estimate the biofilm formation process and ability.Re-sults The A.baumannii strains isolated from respiratory tract were highly resistant.Marjory of A.baumannii strains formed biofilm in vitr0．Non-biofilm-forming strains were more frequently resistant to some antibiotics than their bio-film-forming counterparts.Conclusion A.baumannii respiratory isolates had a inverse correlation between antibiotic resistance and the biofilms formation capability.

关 键 词：呼吸道感染 鲍曼不动杆菌 耐药性 生物被膜 

分 类 号：R446.5[医药卫生—诊断学]