人细小病毒B19的环介导等温扩增快速检测方法的建立  

Establishment of A rapid method for detection of the human parvovirus B19 by a loop-mediated isothermal amplification

作　　者：张彦东[1] 邬巍[1] 马宁[1] 刘涛[1] 王凯[1] 姜振宁[1] 

机构地区：[1]吉林大学第一医院风湿免疫科,吉林长春130021

出　　处：《中国实验诊断学》2015年第8期1249-1251,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的建立一种简单、快速的核酸检测新方法,即环介导等温扩增方法(LAMP)来检测人细小病毒B19的核酸。方法从GenBank中获得B19病毒VP2基因序列,利用PrimerExplorer V3软件在序列保守区域设计LAMP引物,同时对试验中的几个重要参教进行优化,并评价其敏感性和特异性。结果LAMP检测方法的敏感性高于PCR方法,并且对B19病毒的检测具有特异性。结论建立了环介导等温扩增快速检测方法,此方法灵敏性高、特异性强,不仅适用于实验室检测,更能够广泛用于临床检测。Objective To establish a simple and rapid new method for nucleic acid detection,loop-mediated isother-mal amplification （LAMP）to detect human parvovirus B19 nucleic acid.Methods B19 virus of VP2 gene was down-loaded from the GenBank,then primers of LAMP designed using PrimerExplorer V3 software from conserved regions, while the test of several important parameters to optimize teaching and evaluation of its sensitivity and specificity.Re-sults LAMP method is more sensitive than PCR detection method,and specific on the detection of B19.Conclusion Loop-mediated isothermal amplification rapid detection methods has high sensitivity and specificity,not only suitable for laboratory testing,but can be widely used in clinical testing.

关 键 词：人细小病毒B19 环介导等温扩增 核酸检测 

分 类 号：R440[医药卫生—诊断学]