单核细胞CD36表达与强直性脊柱炎  

CD36 expression in monocytes and ankylosing spondylitis

作　　者：覃巍[1] 廉凯[1] 郭青[1] 

机构地区：[1]襄阳市中心医院/湖北文理学院附属医院,湖北省襄阳市441021

出　　处：《中国组织工程研究》2015年第29期4695-4699,共5页Chinese Journal of Tissue Engineering Research

基　　金：湖北省自然科学基金计划(2011CDB318)~~

摘　　要：背景:强直性脊柱炎是一种涉及到慢性全身炎症的自身免疫性疾病,肿瘤坏死因子和白细胞介素6在强直性脊柱炎患者中升高,而肿瘤坏死因子和白细胞介素6等炎症因子可以抑制CD36在单核细胞的表达。目的:分析单核细胞CD36的表达与强直性脊柱炎的关系。方法:纳入84例初诊为强直性脊柱炎的患者和111例健康对照人群,应用流式细胞学技术检测强直性脊柱炎患者和健康人群单核细胞CD36的表达情况,同时,检测两组的生物化学、免疫学、血液常规以及相关炎症因子等指标。结果与结论:两组受试者基本资料比较结果显示,强直性脊柱炎患者单核细胞CD36荧光强度低于健康对照人群(P<0.01)。单核细胞CD36荧光强度水平与C-反应蛋白、血沉、白细胞介素6以及肿瘤坏死因子负相关。另外,单核细胞CD36荧光强度水平与BASDAI评分呈负相关。Logistic回归分析结果显示,血沉、肿瘤坏死因子、白细胞介素6以及单核细胞CD36荧光强度与强直性脊柱炎相关,为强直性脊柱炎患者的危险因素(P<0.05)。结果提示,强直性脊柱炎释放的炎症因子可以下调单核细胞CD36的表达,单核细胞CD36低表达与在强直性脊柱炎存在联系。在临床上,检测单核细胞CD36的表达可能可以作为判断强直性脊柱炎患者机体炎症反应程度和疾病活动性有效的指标。BACKGROUND：Ankylosing spondylitis is an autoimmune disease involved in chronic systemic inflammation. Tumor necrosis factor and interleukin-6 levels increased in patients with ankylosing spondylitis. Inflammatory factors such as tumor necrosis factor and interleukin-6 can suppress CD36 expression in monocytes. OBJECTIVE： To analyze the correlation between CD36 expression in monocytes and ankylosing spondylitis. METHODS：A total of 84 newly diagnosed ankylosing spondylitis patients and 111 healthy individuals were included in this study. CD36 expressions in monocytes in ankylosing spondylitis patients and healthy individuals were tested using flow cytometer; meanwhile, biochemistry, immunology, routine blood examination and related inflammatory markers were determined between the two groups. RESULTS AND CONCLUSION：Results of baseline data in both groups demonstrated that CD36 fluorescence intensity in monocytes was significantly lower in patients with ankylosing spondylitis compared with healthy controls （P 〈 0.01）. CD36 fluorescence intensity in monocytes was negatively correlated with C-reactive protein, erythrocyte sedimentation, interleukin-6 and tumor necrosis factor. In addition, CD36 fluorescence intensity in monocytes was negatively correlated with BASDAI score. Logistic regression analysis showed that erythrocyte sedimentation, interleukin-6, tumor necrosis factor and CD36 fluorescence intensity in monocytes were associated with ankylosing spondylitis, and risk factors for ankylosing spondylitis （P 〈 0.05）. These findings confirm that inflammatory cytokine in patients with ankylosing spondylitis weakened the expression of CD36 in monocytes. There was a remarkable association between low expression of CD36 expression in monocytes and ankylosing spondylitis. CD36 expression of monocytes clinicaly may be considered to be an effective indicator to evaluate inflammation and disease activity in patients with ankylosing spondylitis.

关 键 词：抗原 CD36 脊柱炎 强直性 肿瘤坏死因子α 白细胞介素6 组织构建 骨组织工程 单核细胞CD36 强直性脊柱炎 肿瘤坏死因子 湖北省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]