不同突出类型腰椎髓核中缺氧诱导因子1α的表达  

Expression of hypoxia inducible factor-1 alpha in human lumbar nucleus pulposus of different herniated types

作　　者：刘兴振[1] 傅智轶[1] 沈康平[1] 金文杰[1] 吴玉杰[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院骨科,上海市201900

出　　处：《中国组织工程研究》2015年第29期4700-4704,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:椎间盘内为缺氧的环境,研究发现缺氧诱导因子1在调节缺氧诱导的基因表达中起重要作用。缺氧诱导因子1由α和β两种亚基组成,其中缺氧诱导因子1α决定缺氧诱导因子1的稳定和活性。目的:观察缺氧诱导因子1α在人不同突出类型腰椎间盘突出症髓核中的表达,判断各组之间的关系。方法:取腰椎髓核组织标本60例,41例取自L4-5髓核组织,19例为L5-S1。髓核组织分为突出组、游离组各30例。同时选取腰椎骨折脱位患者的腰椎髓核组织标本10例作为对照组。采用苏木精-伊红、链霉素-过氧化物酶复合物免疫组织化学技术,观察各组突出腰椎髓核组织学变化并测定缺氧诱导因子1α的表达。结果与结论:游离组、突出组和对照组都可观察到缺氧诱导因子1α的表达,分别为(58.2±7.5)%,(27.3±2.3)%和(10.5±4.7)%。游离组缺氧诱导因子1α在髓核的表达显著高于突出组和对照组(P<0.01)。结果提示腰椎间盘突出症髓核组织中缺氧诱导因子1α的表达与突出类型有关,在脱垂游离型中表达最高。BACKGROUND：Under hypoxic environment, hypoxia inducible factor-1 plays an important role in regulation of hypoxia-induced gene expression in the intervertebral disc. Hypoxia-inducible factor-1 consists of α and βsubunits, and which hypoxia inducible factor-1α determines the stability and activity of hypoxia-inducible factor-1. OBJECTIVE： To observe the expression of hypoxia inducible factor-1α in the human lumbar nucleus pulposus of different herniated types and to judge their relationships. METHODS：A total of 60 nucleus pulposus samples were harvested from the lumbar vertebra, including 41 from L4-5 and 19 from L5-S1, and then divided into protruded group and sequestered group, with 30 cases in each group. Meanwhile, another 10 samples of lumbar nucleus pulposus served as controls. Hematoxylin-eosin staining and streptavidin-biotin peroxidase complex immunohistochemical technique were used to observe the expression of hypoxia inducible factor-1α in the human lumbar nucleus pulposus in different groups. RESULTS AND CONCLUSION：The expression level of hypoxia inducible factor-1α was （58.2±7.5）% in the sequestered group, （27.3±2.3）% in the protruded group, and （10.5±4.7）% in the control group, which was significantly higher in the sequestered group than the other two groups （P 〈 0.01）. These findings indicate that the expression of hypoxia inducible factor-1α in the lumbarnucleus pulposus is associated with the herniated types, which is the highest in the prolapse sequestered type.

关 键 词：椎间盘移位 脊髓 缺氧诱导因子1 组织构建 组织工程 椎间盘突出 髓核组织 缺氧诱导因子1Α 

分 类 号：R318[医药卫生—生物医学工程]