莆田黑猪A-FABP基因的群体遗传结构分析  

Analysis of population genetic structure of the A-FABP gene in Putian black pig

作　　者：张金耀[1] 杨建敏[1] 叶炎 黄守婷 何桂娟[1] 彭辉[1] 肖天放[1] 

机构地区：[1]福建农林大学动物科学学院,福州350002

出　　处：《黑龙江畜牧兽医》2015年第8期94-97,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：福建省高校产学合作科技重大项目(2012N5001);科技部科技基础性工作专项项目(2014FY120800)

摘　　要：为了研究莆田黑猪脂肪型脂肪酸结合蛋白(A-FABP)基因的群体遗传结构,试验采用PCRRFLP技术对112头莆田黑猪A-FABP基因的多态性进行检测。结果表明:在A-FABP基因BsmⅠ酶切位点检测到多态性,由2个等位基因控制,定义为B和b,存在3种基因型,即BB、Bb和bb,BB基因型出现的频率较高,基因型频率为0.696 4,B为优势等位基因,基因频率为0.803 6。群体遗传分析结果表明,A-FABP-BsmⅠ位点属于中度多态。卡方检验结果表明,A-FABP-BsmⅠ位点处于Hardy-Weinberg平衡状态(P>0.05)。To study the population genetic structure of adipoeyte fatty acid binding protein （A - FABP） gene in Putian black pig, the polymorphism of the A -FABP genes from 1 t2 Putian black pigs were detected by PCR -RFLP technique. The results showed that the polymorphisms of the A - FABP genes were detected on Bsm I enzyme restriction site, which were controlled by two alleles designated as B and b. The A - FABP gene had three genotypes, namely BB, Bb and bb, and the frequency of gnnotype BB was 0. 696 4 which was higher than other geno- types. B was the dominant allele, and its gene frequency was O. 803 6. The population genetic analysis of the results showed that A - FABP - Bsm I site belonged to a moderate polymorphism. The Chi - square test results showed that A - FABP - Bsm I site was in a Hardy - Weinberg equilibrium （ P 〉0.05 ）.

关 键 词：遗传结构 脂肪型脂肪酸结合蛋白(A-FABP)基因 PCR-RFLP 基因多态性 莆田黑猪 

分 类 号：S828[农业科学—畜牧学] Q786[农业科学—畜牧兽医]