精子质量检测系统在鸡精子密度测定中的应用  

Application of detection system of sperm quality in the determination of chicken sperm density

作　　者：王坤[1,2] 蒋隽[2] 易康乐[1,2] 张佰忠 李昊帮 王庆桥 燕海峰 

机构地区：[1]湖南光大牧业科技有限公司,长沙410131 [2]湖南农业大学,长沙410128 [3]湖南天心黄鸡育种有限公司,长沙410143

出　　处：《黑龙江畜牧兽医》2015年第8期106-108,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家国际科技合作专项(2013DFG31810);湖南省科技计划项目(2014WK2008);长沙市科技计划项目(K1307134-24)

摘　　要：为了明确精子质量检测系统在鸡精子密度测定中的适用性,试验采用精子质量检测系统与计数板法测定黑丝羽乌骨鸡及其杂交鸡公鸡精液精子密度,比较了精子质量检测系统在鸡精子密度测定中的重复性和准确性。结果表明:该系统的重复性和一致性较好,10倍与20倍物镜检测结果无显著差异(P>0.05);该检测系统准确性较好,与计数板法测得的结果比较无显著差异(P>0.05),可以在实际研究与生产中应用。To clarify the applicability of detection System of sperm quality in the determination of chicken sperm density, the sperm densities in the cock semen from black silkies and their hybrids were detected using the detection system of sperm quality and the counting chamber method, and then the repeatability and accuracy of the detection system of sperm quality in the determination of chicken sperm density were compared. The results showed that the repeatability and consistency of the detection system were better in the determination of chicken sperm density. There was no significant difference （ P 〉 0.05 ） between the results detected by using 10 - fold and 20 - fold objectives. The accuracy of the de- tection system was also better in the determination of chicken sperm density, and there was also no significant difference （ P 〉 O. 05 ） between the results detected by the detection system of sperm quality and the counting chamber method. The results indicate the detection system of sperm quality can be applied in actual research and production.

关 键 词：鸡 精子质量检测系统 计数板法 密度 精液 

分 类 号：S831[农业科学—畜牧学] S821.35[农业科学—畜牧兽医]