蔬菜土传病害生防木霉菌株资源的筛选及其防治效果评价  

Screening and Bio-control Effect Assessment of Trichoderma Strains against 3 Soilborn Vegetable Pathogens

作　　者：朱萍萍[1] 凌健[1] 席亚东[1] 陈国华[1] 茆振川[1] 杨宇红[1] 谢丙炎[1] 

机构地区：[1]农业部蔬菜遗传改良重点开放实验室,中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《中国蔬菜》2015年第8期28-33,共6页China Vegetables

基　　金：国家公益性行业(农业)科研专项(201303014);国家大宗蔬菜产业技术体系建设项目(CARS-25-B-01);农业部园艺作物遗传改良重点开放实验室项目

摘　　要：采用平板对峙接种法和温室盆栽试验,测定18种木霉共175个菌株对黄瓜枯萎病菌、辣椒疫霉病菌和番茄青枯病菌3种不同类型的蔬菜土传病害病原菌的防效,筛选生防木霉资源。试验结果表明：拮抗试验中,拮抗黄瓜枯萎病菌的木霉菌株有50株,抑制率达70.0%~86.3%;拮抗辣椒疫霉病菌的木霉菌株43株,抑制率达70.4%~88.7%。盆栽试验中,筛选出番茄青枯病的生防菌株32株,防效达50.0%~92.7%。进一步对在拮抗试验中对黄瓜枯萎病菌抑制率达75%以上的其中13株木霉菌进行盆栽防效评价,获得防效超过60%的高效菌株2株,分别为T52（深绿木霉）和32080（平菇木霉）。This paper had screened the bio-control resources of 18 Trichoderma species, including 175 strains, against 3 kinds of soil-born vegetable pathogens as Fusarium oxysporum f. sp. cucumebrium Owen, Phytophthora capsici and Ralstonia solanacearum through confront culture experiments and green house potted plant experiments. The results indicated that through confront culture experiments, we have screened out 50 Trichoderma strains with inhibitation rate as 70.0%-86.3% against Fusarium oxysporum f. sp. cucumebrium Owen, and 43 Trichoderma strains with inhibitation rate as 70.4%-88.7% against Phytophthora capsici. And the potted plant experiments have screened out 32 Trichoderma strains with bio-control efficacy of 50.0%-92.7% against Ralstonia solanacearum. We further assessed the bio-control efficacy of 13 Trichoderma strains with antagonistic inhibitation rate higher than75% by potted plant experiment, and found 2 Trichoderma

关 键 词：木霉 黄瓜枯萎病菌 辣椒疫霉病菌 番茄青枯病菌 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.3[农业科学—植物保护]