紫杉醇对大鼠尿道成纤维细胞增殖和胶原生成的影响  

Effects of paclitaxel on the proliferation and collagen production of urethral fibroblasts in male rats

作　　者：翟晓强[1] 张刚[2] 付德来[1] 李和程[1] 李建平[1] 种铁[1] 

机构地区：[1]西安交通大学第二附属医院泌尿外科,陕西西安710004 [2]四川省达州市中心医院泌尿外科,四川达州635000

出　　处：《现代泌尿外科杂志》2015年第8期580-583,共4页Journal of Modern Urology

摘　　要：目的探讨紫杉醇对大鼠尿道成纤维细胞增殖和胶原生成的影响。方法组织块接种法培养大鼠尿道成纤维细胞。以不同浓度紫杉醇(0、5、10、20、40、80mg/L)处理尿道成纤维细胞,MTT法检测成纤维细胞增殖能力,羟脯氨酸法检测培养液中成纤维细胞Ⅰ型和Ⅲ型胶原生成,RT-PCR检测成纤维细胞中TGF-β1、Ⅰ型胶原、Ⅲ型胶原、TIMP-1等mRNA表达水平。结果与对照组相比,实验组不同浓度紫杉醇均对成纤维细胞的增殖有抑制作用。各组间细胞抑制率差异有统计学意义(P<0.05)。紫杉醇浓度越高,抑制作用越强。实验组培养液中胶原浓度、每十万细胞胶原浓度比对照组显著降低,差异具有统计学意义(P<0.05)。实验组尿道成纤维细胞TGF-β1、Ⅰ型胶原、Ⅲ型胶原和TIMP-1的mRNA表达量均低于对照组(P<0.05)。结论紫杉醇可抑制大鼠尿道成纤维细胞增殖、胶原生成以及细胞中TGF-β1、Ⅰ型胶原、Ⅲ型胶原和TIMP-1的mRNA表达,紫杉醇在预防、治疗尿道狭窄形成中的作用值得进一步研究。Objective To investigate the effects of paclitaxel on the proliferation and collagen production of urethral fibroblasts in male rats. Methods Urethral fibroblasts were cultivated with tissue inoculation, which were then treated with different concentrations of paclitaxel （0mg/L, 5 mg/L, 10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L）. Cell proliferation was evaluated with MTT assay. The production of collagen I and Ⅲ were assessed with hydroxyproline assay. The mRNA expressions of TGF- β1, collagen I , Ⅲ, and TIMP-1 in the fibroblasts were evaluated with RT-PCR. Results Compared with control group, cell proliferation was significantly inhibited by paclitaxel in the treatment groups. The inhibition rate was dose-dependently different between each group （P〈0.05）. In the treatment groups, collagen concentration and collagen expression per 105 cells were lower than that in control, and the difference was statistically significant （P〈0.05）. Meanwhile, the mRNA expressions of TGF-β1, collagen I, collagen Ⅲ and TIMP-1 were significantly lower compared with control （P〈0.05）. Conclusions Paclitaxel could significantly inhibit the proliferation and collagen production of rat urethra fibroblasts in vitro. Moreover, paclitaxel could reduce mRNA expressions of TGF-β1, collagen I, collagen III and TIMP-1. Further studies are deserved to investigate the effect of paclitaxel on the prevention and treatment of urethral stricture.

关 键 词：紫杉醇 尿道狭窄 成纤维细胞 瘢痕 

分 类 号：R34[医药卫生—基础医学]