不同产地丁公藤药材的HPLC特征图谱对比研究  

Comparative Study on HPLC Characteristic Spectrum of Erycibe obtusifolia from Different Habitats by HPLC

作　　者：文婧[1] 孙洪章[1] 卢静华[1] 

机构地区：[1]辽宁医学院研究生学院,辽宁锦州121000

出　　处：《中国药房》2015年第24期3417-3419,共3页China Pharmacy

基　　金：辽宁省教育厅科研项目(No.L2010332)

摘　　要：目的:建立并比较不同产地丁公藤药材的高效液相色谱特征图谱。方法:采用高效液相色谱法。色谱柱为Hypersil ODS C18,流动相为乙腈-0.1%磷酸溶液,流速为1.0 ml/min,检测波长为347 nm,柱温为30℃,进样量为20μl。收集不同批次的12批样品进行测定,建立12批药材的特征图谱,并利用《中药色谱指纹图谱相似度评价系统(2004版)》对12批样品的相似度进行评价。结果:从丁公藤药材的特征图谱中分析得出10个共有峰,其中3号峰为东莨菪苷,4号峰为绿原酸,6号峰为东莨菪内酯。经分析,其中10批药材的相似度在0.90以上,2批药材的相似度在0.90以下。结论:该方法简便、准确、重复性好,可为有效地控制丁公藤药材的内在质量标准提供科学依据。OBJECTIVE： To establish and compare the HPLC characteristic Spectrum Erycibe obtusifolia from different habitats. METHODS： HPLC was performed on the column of Hypersil ODS C18 with the mobile phase of acetonitrile -0.1% phosphoric acid at flow rate of 1.0 ml/min, detection wavelength was 347 nm, the colunm temperature was 30 ℃ and the sample size was 20 μ 1. 12 different batches of samples were determined and the characteristic spectrums of those were established. TCM fingerprint evaluation software （2004 edition）was used to evaluate the similarity of 12 batches of samples. RESULTS： 10 characteristic peaks were identified in the characteristic spectrum of E. obtusifolia. Peak 3 was scopolamine glycosides, peak 4 was chlorogenic acid and peak 6 was scopoletin. The similarities of 10 batches of samples were proved to be higher than 0.90 and 2 batches were proved to be less than 0.90. CONCLUSIONS： The method is simple, accurate and reproducible, and can provide scientific evidence for effectively controlling the internal quality standards.

关 键 词：丁公藤 特征图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]