多波长RP-HPLC法同时测定参芍口服液中5种成分的含量  

Simultaneous Determination of 5 Components in Shenshao Oral Liquid by Multi-wavelength RP-HPLC Method

作　　者：付连浩 张熙洁[1] 王绍志[1] 刘晓红[1] 

机构地区：[1]唐山市工人医院,河北唐山063000

出　　处：《中国药房》2015年第24期3423-3426,共4页China Pharmacy

基　　金：河北省自然科学基金石药集团医药联合研究基金资助项目(No.C2011105042)

摘　　要：目的：建立同时测定参芍口服液中丹参素、原儿茶醛、芍药苷、阿魏酸、丹酚酸B含量的方法。方法：采用反相高效液相色谱法。色谱柱为Eclipse XDB C18,流动相为0.5%磷酸水溶液-甲醇-乙腈（梯度洗脱）,流速为1.0 ml/min,检测波长为280、230、320nm,柱温为30℃,进样量为10μl。结果：在此色谱条件下,5种成分可完全分离,丹参素、原儿茶醛、芍药苷、阿魏酸、丹酚酸B的质量浓度分别在24~384、1.25~20、40.5~648、1.5~24、145~2 320μg/ml范围内与各自峰面积呈良好的线性关系（r=0.999 9、0.999 9、0.999 8、0.999 9、0.999 9）;精密度、稳定性、重复性试验的RSD≤2.2%;平均加样回收率分别为100.7%、100.0%、99.6%、100.3%、99.3%,RSD分别为1.23%、2.19%、0.87%、1.11%、2.46%（n=9）。结论：该方法专属性强,精密度、准确度好,可用于测定参芍口服液中5种成分的含量。OBJECTIVE： To establish a method for simultaneous determination of tanshinol, protocatechuic aldehyde, paeoniflorin, ferulic acid and salvianolic acid B in Shenshao oral liquid. METHODS ： RP-HPLC was performed on the column of Eclipse XDB C18 with mobile phase of 0.5% phosphoric acid-methanol-acetonitrile （gradient elution） at the flow rate of 1.0 ml/min, the detection wavelength was 280, 230 and 320 nm, column temperature was 30 ℃ and volume was 10 μl. RESULTS： Under the chromatographic conditions, 5 kinds of components could be completely separated, the linear range of tanshinol, protocatechuic aldehyde, paeoniflorin, ferulic acid and salvianolic acid B were respectively 24-384 μg/ml（r=0.999 9）, 1.25-20 μ/ml（r=0.999 9）, 40.5-648 μg/ml （r=0.999 8）, 1.5-24 μg/ml（r=0.999 9）, 145-2 320 μg/ml（r=0.999 9）; RSDs of precision, stability and reproducibility tests were no more than 2.2%; the average recovery was respectively 100.7% （RSD=1.23%, n=9）, 100.0% （RSD= 2.19% ,n=9） ,99.6% （RSD=0.87% ,n=9）, 100.3% （RSD=1.11% ,n=9） and 99.3% （RSD=2.46% ,n=9）. CONCLUSIONS： The method is specific with good precision and reproducibility, and can be used for the content determination of 5 components in Shenshao oral liquid.

关 键 词：多波长反相高效液相色谱法 参芍口服液 丹参素 原儿茶醛 芍药苷 阿魏酸 丹酚酸B 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]