大熊猫胃肠道神经肽Y和长型瘦素受体的表达  

Expression of neuropeptide Y and long leptin receptor in gastrointestinal tract of giant panda

作　　者：罗启慧[1] 唐秀莹[1] 陈正礼[1] 汪开毓[1] 王承东[2] 李德生[2] 李才武[2] 

机构地区：[1]四川农业大学动物医学院动物疾病与人类健康中心四川省重点实验室,四川雅安625014 [2]中国保护大熊猫研究中心濒危动物遗传与育种四川省重点实验室,四川雅安625014

出　　处：《生物工程学报》2015年第8期1175-1183,共9页Chinese Journal of Biotechnology

基　　金：中国保护大熊猫研究中心大熊猫俱乐部项目;教育部"长江学者和创新团队发展计划"创新团队项目(No.IRT0848);四川省青年科技创新研究团队项目(No.2013TD0015)资助~~

摘　　要：为了观察神经肽Y(NPY)和瘦素长型受体(OB-Rb)在大熊猫胃肠道的表达分布,并探讨其功能。本实验选用中国保护大熊猫研究中心濒危动物繁殖与保护遗传四川省重点实验室提供的3例大熊猫胃肠组织样品,采用HE和免疫组化SABC法进行组织学、NPY及OB-Rb蛋白的表达研究。HE染色结果显示3例大熊猫胃肠道组织学结构完整,主要表现在单细胞和多细胞黏液腺丰富、小肠绒毛较长,肠道黏膜肌层与肌肉层较厚等。免疫组化方法观察到NPY和OB-Rb阳性产物广泛分布于大熊猫的胃肠道中。NPY阳性神经纤维呈串珠状或点状排列,主要位于黏膜下神经丛和肌间神经丛内,前者阳性神经纤维数量较多。NPY阳性细胞主要分布于黏膜层和肠腺,多呈椭圆形、多边形等。OB-Rb阳性产物主要分布在黏膜层,且在固有层内有大量阳性细胞分布。表明NPY和OB-Rb在大熊猫肠道中的广泛表达,为研究NPY和OB-Rb影响肠道的生长发育、消化吸收、免疫等多种功能奠定了基础。To study the expression and distribution of neuropeptide Y （NPY） and long leptin receptor （OB-Rb） in the gastrointestinal tract of giant panda, samples of three animals were collected from the key laboratory for reproduction and conservation genetics of endangered wildlife of Sichuan province, China conservation and research center for the giant panda. Paraffin sections of giant panda gastrointestinal tissue samples were observed using hematoxylin-eosin staining （HE） and strept actividin-biotin complex immunohistochemical staining （IHC）. The results show that the intestinal histology of three pandas was normal and no pathological changes, and there were rich single-cell and multi-cell mucous glands, long intestinal villi and thick muscularis mucosa and muscle layer. Positive cells expressing NPY and OB-Rb were widely detected in the gastrointestinal tract by IHC methods. NPY positive nerve fibers and neuronal cell were widely distributed in submucosal plexus and myenteric plexus, especially in the former. They were arranged beaded or point-like shape. NPY positive cells were observed in the shape of ellipse and polygon and mainly located in the mucous layer and intestinal glands. OB-Rb positive cells were mainly distributed in the mucous layer and the laminae propria, especially the latter. These results confirmed that NPY and OB-Rb are widely distributed in the gut of the giant panda, which provide strong reference for the research between growth and development, digestion and absorption, and immune function.

关 键 词：大熊猫 神经肽Y 瘦素长型受体 免疫组化 胃肠道 

分 类 号：S865.3[农业科学—野生动物驯养]