五行音乐联合理虚解郁方对慢性疲劳综合征大鼠模型的行为学影响  

Effect of Five- element Music Therapy Combined with Lixu Jieyu Formula on Behavioristics of CFS Rats

作　　者：吴丽丽[1] 张振贤[1] 张烨[1] 董书桂 李娜[2] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院中医内科,上海200437 [2]上海中医药大学,上海201203

出　　处：《辽宁中医杂志》2015年第8期1556-1559,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81173227);上海市卫生局项目(30304115240);国家中医药管理局十二五重点学科"治未病学科"项目;上海市三年中医行动计划项目(ZYSNXD-YL-CTXK);岳阳医院慢性疲劳综合征重点专科建设项目

摘　　要：目的:观察五行音乐联合中药理虚解郁方对慢性疲劳综合征大鼠行为学的影响。方法:将清洁级SD雄性大鼠78只随机分为正常对照组、模型组、理虚解郁方组、音乐组(按五行音乐:宫商角徵羽五调分为5组),中药联合音乐治疗组(按五行音乐:宫商角徵羽五调分为5组,简称音药组)每组6只。给予复合因素造模(慢性束缚、冷水游泳、夹尾激怒法),同时正常组、模型组予生理盐水2 m L,理虚解郁方组及音药组予中药2 m L灌胃,音乐及音药组同时接受五行音乐治疗,连续30 d,观察治疗前后大鼠行为学(旷场实验、鼠尾悬挂实验、力竭游泳实验)的变化。结果:1与正常组比较,模型组大鼠穿格次数、直立次数、挣扎次数减少,静止时间延长,力竭游泳时间明显缩短,差异有统计学意义(P<0.05);2与模型组比较,中药组力竭游泳时间明显延长,挣扎次数、直立次数、穿格次数增加,静止时间缩短,其差异均有统计学差异(P<0.05)。各音乐组力竭游泳时间明显延长;且角调组挣扎次数增加、静止时间缩短,其差异有统计学意义(P<0.05);各音药组力竭游泳时间明显延长,药徵组直立次数、穿格次数、挣扎次数均增加,静止时间缩短;药角组穿格次数、及挣扎次数增加,药宫组穿格次数增加,药商组静止时间缩短,药羽组穿格次数、挣扎次数均增加,静止时间缩短,差异均有统计学意义;3各组间比较,中药组穿格次数较药宫组、药商组、药羽组增加;药角组、药徵组较其对应单纯音乐组穿格次数增加,药徵组直立次数增加,其差异有统计学意义。结论:五行音乐联合中药理虚解郁方对于缓解大鼠的躯体及精神疲劳有较为明显的疗效,角调、徵调提示有初步增效作用。Objective ： To observe the effect of five - element music therapy combined with Lixu Jieyu Formula on behavioristics of CFS rats made with multiple stimuli. Methods ： 78 SD male rats were randomly divided into 5 groups ： control group, model group,Lixu Jieyu group, Five -element music group （divided into 5 groups by different kinds of music） and the combined group （divided into 5 groups by different kinds of music） ,6 rats in each group. The CFS rats models were made with multiple stimuli, including chronic restraint, swimming in cold water and provoked by catching tails. The rats in the control group and model group were given normal saline 2 mL. Lixu Jieyu group and the combined group were given Lixu Jieyu Formula 2 mL. Five - element music group and the combined group were given five - element music therapy for 30 days. We observed the ratsbehavioristic changes by Open 2 field test,tail hanging test and exhausting swimming test. Results：（1）Compared with the control group,the model group manifested a series of abnormal variations in Open - field test, tail hanging test and exhausting swimming test （ P 〈 0. 05 ）. （2）Lixu Jieyu Formula can improve the times of crossing lattice in Open - field test, shorten the time of static, improve the times of flounces and vertical and prolong the time of swimming till exhaustion （ P 〈 0.05 ）. Each music group can prolong the time of swimming remarkably and Jiao - music can improve the times of flounces and shorten the time of being static （ P〈 0. 05 ）. Each combined group can prolong the time of swimming remarkably and manifested a series of abnormal variations by different five - el- ement music therapies. （3）Compared among groups, the times of crossing lattice of Lixu Jieyu group were more than that combinedwith Gong or Shang or Yu music. Compared with the single music group, the times of crossing lattice of that combined with Jiao or Zheng - music were more. The times of vertical were more in that combined with Zheng - music �

关 键 词：理虚解郁方 五行音乐 慢性疲劳综合征 动物实验 行为学 

分 类 号：R332[医药卫生—人体生理学]