小鼠微量圆形精子细胞冷冻保存方法研究  

An optimal method for cryopreservation of microamount round spermatids of the mouse

作　　者：姜宏[1] 王丽 汪存利[1] 

机构地区：[1]解放军第105医院生殖中心,安徽合肥230031 [2]安徽省第二人民医院妇产科,安徽合肥230031

出　　处：《中华男科学杂志》2015年第8期698-701,共4页National Journal of Andrology

基　　金：安徽省自然科学基金(090413271X);南京军区科技创新项目(09MA040)~~

摘　　要：目的:探讨小鼠微量圆形精子细胞的冷冻方法和条件。方法:比较玻璃化冷冻和常规慢冷冻,不同浓度冷冻保护剂(5%、7%、9%甘油)及不同平衡时间(0、15、30、45、60 min)对体外培养所获微量小鼠圆形精子细胞的冷冻效果。结果:玻璃化冷冻和常规慢冷冻在7%甘油中平衡时间30 min均可获得较高的细胞复苏率,且玻璃化冷冻小鼠圆形精子细胞的复苏率显著高于常规慢冷冻方法[(72.9±15.4)%vs.(58.2±17.7)%,P<0.05]。结论:采用玻璃化冷冻方法,在7%浓度的甘油保护剂中平衡30 min,可获得较满意的微量圆形精子细胞冷冻效果。Objective： To search for an optimal protocol and freezing conditions for the cryopreservation of microamount round spermatids of the mouse. Methods： We compared the survival rates of frozen-thawed microamount round spermatids of the mouse achieved by vitrification or standard slow freezing with different concentrations of glycerol （5,7, or 9% ） and different lengths of equi- librium time （0, 15, 30, 45, or 60 rain）. Results： Under the conditions of 7% glycerol and 30 rain equilibrium, both vitrification and standard slow freezing achieved high survival rates of spermatids, and the former obtained an even higher rate than the latter （[72.9±15.41% vs [58.2 ±17.71%, P〈0.05）. Conclusion： A high rate of frozen-thawed microamount round spermatids of the mouse can be achieved by vitrification under the conditions of 7% glycerol and 30 min equilibrium.

关 键 词：冷冻保存 园形精子细胞 玻璃化冷冻 甘油 

分 类 号：R714.8[医药卫生—妇产科学]