维药毛菊苣根-种子不同比例配伍的90%乙醇提取物对小鼠急性肝损伤的保护作用及其机制  

Protective effects and mechanism of 90% ethanol extract of different roots and seeds compatibility proportion of Cichorium glandulosum on acute liver injuries in mice

作　　者：杨飞[1] 罗玉琴[2] 薛桂蓬 杨伟俊 

机构地区：[1]石河子大学校医院,新疆石河子832002 [2]中国科学院干旱区植物资源化学重点实验室,中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [3]新疆维吾尔药重点实验室,新疆维吾尔自治区药物研究所,新疆乌鲁木齐830004

出　　处：《中国医院药学杂志》2015年第15期1352-1356,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:81303307)

摘　　要：目的:研究毛菊苣根-种子药对不同比例配伍90%乙醇提取物的化学指纹谱与肝损伤保护作用的相关性,探索维药处方中"药对"的配伍机制。方法:将毛菊苣的根和种子按6个质量比例(1∶0,0∶1,1∶1,1∶2,1∶3,2∶1)配伍,用90%乙醇提取,得到6个提取物;观察不同提取物对四氯化碳(CCl4)致小鼠急性化学性肝损伤模型,以及小鼠尾静脉注射卡介苗(BCG)和脂多糖(LPS)造成急性免疫性肝损伤模型,血清中天冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT)水平的影响及肝脏组织病理改变。采用高效液相色谱法分析各提取物的化学谱图,观察与药效相关的化学物质变化。结果:毛菊苣根-种子以质量比1∶2配伍能显著降低CCl4致小鼠急性化学性肝损伤引起的血清AST(P<0.01)和ALT(P<0.01)升高,对BCG+LPS致急性免疫性肝损伤模型血清中AST(P<0.05)和ALT(P<0.01)也有明显的改善作用;另外,毛菊苣根-种子以质量比1∶3配伍对CCl4致小鼠急性化学性肝损伤引起的血清AST(P<0.01)和ALT(P<0.05)升高,以及对BCG+LPS致急性免疫性肝损伤引起的血清AST(P<0.05)和ALT(P<0.01)升高也有明显的改善作用。化学谱图中的色谱峰与提取物改善CCl4致小鼠急性化学性肝损伤引起的血清AST和ALT变化呈正相关。结论:用现代研究方法证实了维药毛菊苣不同药用部位配伍的科学性,进一步从化学角度探索了毛菊苣根-种子肝保护作用的临床经典比例1∶2和1∶3配伍的可能机制。OBJECTIVE To study correlation of chemical fingerprint spectrum and hepatoprotective effects of extracts of different compatibility proportion of roots-seeds from Cichorium glandulosurn on acute liver injury in mice, and to objectively explore its compatibility mechanism. METHODS Different compatibility proportions of roots and seeds （1 ： 0, 0 ： 1, 1 ： 3, 1 ： 2, 1 ： 1, 2：1） of C. glandulosum were extracted with 90; ethanol, then 6 extracts were obtained. Extracts above were evaluated in vivo using two experimental models, carbon tetrachloride （CCl4） and Bacillus Calmette Guerin （BCG） plus lipopolysaccharide （LPS）-induced acute hepatotoxicity in mice. Aspartate aminotransferase （AST） and alanine aminotransferase （ALT） in serum and liver tissues were investigated, respectively. Chemical spectra were analyzed using HPLC to explore chemicall mechanism. RESULTS Pretreatment with 90 % ethanol extract of roots and seeds ratio 1：2 for seven days obviously reduced impact of CCl4 toxicity on serum markers of liver damage, with significantly different AST （P〈0. 01） and ALT （P〈0. 01）, respectively. Further, protective activity was reconfirmed against BCG/LPS-induced injury and serum enzymatic levels were elevated obviously, with significantly different AST （PM0. 05） and ALT （P〈0. 01）. Meanwhile, Pretreatment with 90% ethanol extract of roots and seeds ratio 1 ： 3 reduced impact of CCl4 toxicity on serum markers of liver damage, with significantly different AST （P〈0. 01） and ALT （P〈0. 05）, which was reconfirmed against BCG/LPS-induced injury and elevated serum enzymatic lev els, with significantly different AST （P〈0. 05） and ALT （P〈0. 01）. The chromatogram showed a marked correlation with pharmacodynamic effects. CONCLUSION Scientificity of compatibility of different medicinal parts of C. glandulosum have been proved using modern research method. The rationality of hepatoprotective classical compatibility proportions in 1 ： 2 and 1 ： 3 o

关 键 词：毛菊苣 根-种子药对 配伍比例 肝损伤保护 药效相关性 

分 类 号：R969[医药卫生—药理学]