贝那普利对体外高血压患者内皮祖细胞功能的影响及时间依赖性研究  

Effects of benner pury on the function of endothelial progenitor cells in vitro from patients with hypertension in time dependence manner

作　　者：李永东[1,2] 温慧华[1] 连瑞珍[1] 阿拉坦高勒[2] 

机构地区：[1]内蒙古医科大学第三附属医院心内科,内蒙古包头014010 [2]内蒙古大学生命科学学院

出　　处：《临床心血管病杂志》2015年第8期818-822,共5页Journal of Clinical Cardiology

基　　金：内蒙古自治区自然科学基金(No:2011MS1150)

摘　　要：目的:探讨贝那普利对体外高血压患者内皮祖细胞增殖、迁移、黏附、凋亡、氧化应激能力影响及时间依赖性。方法:选取符合《中国高血压防治指南2010》的诊断标准的1级高血压病患者,用密度梯度离心法分离单个核细胞,acLDL-DiI和、FITC-lectin染色程双阳性细胞被认为是正在分化的内皮祖细胞(EPC)。对照组为年龄、性别与研究组相匹配的健康体检者。将培养5d的EPC用于研究分别在贝那普利不同干预时间点收获细胞,检测EPC的增殖、迁移、黏附能力及凋亡、氧化应激指标。结果:1与对照组比较高血压组外周血EPS增殖、迁移、黏附能力均明显降低(P<0.01),EPS凋亡率明显增加,氧化应激反应明显增高(P<0.01)。2贝纳普利可以显著改善EPC增殖、迁移、黏附能力及凋亡和氧化应激反应,且呈时间依赖性(P<0.05)。结论:贝那普利可以改善高血压患者EPC增殖、迁移、黏附、凋亡和氧化应激能力,并呈时间依赖性。Objective：To investigate the effect of Benner Pury on endothelial progenitor cells proliferation, mi- gration, adhesion, apoptosis, oxidative stress in vitro from patients with hypertension and its time dependence. Method：The patients who were diagnosed as hypertension grade 1, according to the standard ＂Guidelines for Pre- vention and Treatment of Hypertension＂ were enrolled. Mononuclear cells were isolated by density gradient cen- trifugation and stained with fluorescence chemical acLDL-DiI and FITC-leetin. Double staining positive cells were considered as the differentiation of EPC. The control group were healthy subjects matched with study group in age, gender. EPC cultivated for 5 days were used for study. Cells harvested at different set times under the stimu- lation of Benner Pury, and the EPC proliferation, migration, adhesion ability, apoptosis and oxidative stress indicators were detected respectively. Result： 1Compared with the control group, peripheral blood EPS cell prolif- eration, migration, adhesion ability were obviously decreased in hypertension group（P〈0.01）, EPS apoptosis rate and oxidative stress response was significantly increased（P〈0.01）.2Benner Pury significantly increased the EPC proliferation, migration, adhesion ability and improve apoptosis and oxidative stress reaction, and in a time dependent manner . Conclusion..Benner Pury can improve the EPC proliferation, migration, adhesion, apoptosis and oxidative stress in patients of hypertension ,and in a time dependent manner.

关 键 词：高血压 内皮祖细胞 增殖 迁移 黏附 凋亡 氧化应激 

分 类 号：R544.1[医药卫生—心血管疾病]