适当质量浓度黄芪注射液可增强骨髓基质细胞的表面黏附性  

Radix Astragali injection at appropriate concentrations enhances surface adhesion of bone marrow stromal cells

作　　者：王彩霞[1] 任明姬[2] 崔明玉[2] 

机构地区：[1]内蒙古医科大学第二附属医院麻醉科,内蒙古自治区呼和浩特市010030 [2]内蒙古医科大学组织胚胎学教研室,内蒙古自治区呼和浩特市010059

出　　处：《中国组织工程研究》2015年第28期4445-4449,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究发现很多益气补血类中药均是通过影响骨髓基质细胞分泌一些细胞因子来促进造血干细胞的分化增殖,或者促进骨髓基质细胞和造血干细胞的黏附而发挥作用。目的:观察黄芪注射液对小鼠骨髓基质细胞表面细胞间黏附分子1、血管细胞间黏附分子1表达的影响。方法:采用全骨髓贴壁细胞分离筛选法培养小鼠骨髓基质细胞,倒置相差显微镜、苏木精-伊红染色光镜及透射电镜观察小鼠骨髓基质细胞的形态。MTT法检测黄芪注射液促进骨髓基质细胞增殖的最佳浓度。流式细胞术检测黄芪注射液干预后小鼠骨髓基质细胞表面细胞间黏附分子1、血管细胞间黏附分子1的表达。结果与结论:倒置显微镜和光镜观察到骨髓基质细胞呈贴壁生长,细胞呈梭形或不规则形态,有突起;透射电镜观察到细胞内细胞器丰富,如粗面内质网、分泌小泡、线粒体等。黄芪注射液质量浓度为400和600 mg/L时可促进小鼠骨髓基质细胞增殖(P<0.05),且两种剂量组间比较差异无显著性意义。600 mg/L黄芪注射液可增加小鼠骨髓基质细胞表面细胞间黏附分子1、血管细胞间黏附分子1蛋白表达。结果表明适当质量浓度黄芪注射液能够增强基质细胞的黏附性,对造血微环境有改善作用。BACKGROUND：Several studies have demonstrated that many kinds of Chinese herbs for benefiting vital energy and enriching the blood secrete some cytokines by influencing bone marrow stromal cel s to promote the differentiation and proliferation of hemopoietic stem cel s or exert effects by promoting the adhesion of bone marrow stromal cel s and hemopoietic stem cel s. OBJECTIVE：To investigate the effects of Radix Astragali injection on the expression of intercel ular adhesion molecule 1 and vascular cel adhesion molecule 1 on the surface of mouse bone marrow stromal cel s. METHODS：Mouse bone marrow stromal cel s were isolated by adherent culture of whole bone marrow. The morphology of mouse bone marrow stromal cel s were observed using inverted phase microscopy, hematoxylin-eosin staining, optical microscopy and transmission electron microscopy. The optimal concentration at which Radix Astragali injection exhibited the strongest effects on promoting the proliferation of bone marrow stromal cel s was detected using MTT assay. Expression of intercel ular adhesion molecule 1 and vascular cel adhesion molecule 1 on the surface of mouse bone marrow stromal cel s was detected using flow cytrometry after intervention by Radix Astragali injection. RESULTS AND CONCLUSION：Inverted phase microscopy and optical microscopy showed that bone marrow stromal cel s adhered to the wal of culture flask, exhibited a shuttle-shaped or irregular appearance with processes. Through transmission electron microscopy, organel es were abundant, such as rough endoplasmic reticulum, secretory vesicle, and mitochondria. Radix Astragali injection at 400 and 600 mg/L significantly promoted the proliferation of mouse bone marrow stromal cel s （P〈0.05） and there was no significant difference in the cel proliferation between these two concentrations. Radix Astragali injection at 600 mg/L increased the expression of intercel ular adhesion molecule 1 and vascular cel adhesion molecule 1 on the surface of mouse bone marrow stromal cel s.

关 键 词：干细胞 骨髓干细胞 黄芪注射液 小鼠骨髓基质细胞 细胞间黏附分子1 血管细胞间黏附分子1 

分 类 号：R318[医药卫生—生物医学工程]