血管内皮生长因子165基因促进人脂肪间充质干细胞的增殖  

Vascular endothelial growth factor 165 promotes proliferation of human adipose-derived mesenchymal stem cells

作　　者：王钰[1] 朱志图[2] 陈峻江[3] 

机构地区：[1]辽宁医学院附属第一医院临床生物样本中心,辽宁省锦州市121000 [2]辽宁医学院附属第一医院肿瘤科,辽宁省锦州市121000 [3]辽宁医学院附属第一医院学科科研处,辽宁省锦州市121000

出　　处：《中国组织工程研究》2015年第28期4485-4492,共8页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省科技厅科技计划项目(2013225305)~~

摘　　要：背景:目前自体脂肪移植已广泛运用于美容整形和修复创伤导致的软组织缺损的修复,有研究表明,移植后1年移植脂肪存活率为20%-80%,因此,在移植后的早期及时、充分的血供建立,对于移植脂肪的存活是非常重要的。目的:观察血管内皮生长因子165转染人脂肪间充质干细胞的增殖情况。方法:体外传代培养人脂肪间充质干细胞,将重组血管内皮生长因子165基因入腺病毒液和空病毒液转染至脂肪间充质干细胞内,分别设为实验组和对照组,另设正常培养的细胞为空白组。结果与结论:RT-PCR,Western blot,MTT检测显示,与对照组和空白组相比,实验组血管内皮生长因子165 m RNA和蛋白的表达及细胞增殖均增高(P<0.05)。结果证实,腺病毒承载的血管内皮生长因子165基因转染脂肪间充质干细胞后不仅可以持续的表达目的蛋白,同时也能显著促进脂肪间充质干细胞的增殖。BACKGROUND：Autologous fat transplantation has been widely used in soft tissue defect repair and cosmetic surgery, and the 1-year transplant survival rate is 20%-80%. Therefore, the establishment of timely and adequate blood supply at early period after transplantation is very important for the survival of transplanted fat tissues. OBJECTIVE：To observe the proliferation of human adipose-derived mesenchymal stem cel s transfected with vascular endothelial growth factor 165. METHODS：Human adipose-derived mesenchymal stem cel s were subcultured in vitro. Recombinant adenovirus carrying vascular endothelial growth factor 165 and blank virus were respectively transferred into adipose-derived mesenchymal stem cel s. Cel s cultured normal y served as blank group. RESULTS AND CONCLUSION：Compared with the control and blank groups, the expressions of vascular endothelial growth factor 165 mRNA and protein were higher in the experimental group （P〈0.05）. Experimental findings suggest that the recombinant adenovirus carrying vascular endothelial growth factor 165 transferred into adipose-derived mesenchymal stem cel s cannot only maintain the expression of target protein but also obviously promote the proliferation of adipose-derived mesenchymal stem cel s.

关 键 词：组织工程 干细胞 细胞增殖 病毒 脂肪干细胞 血管内皮生长因子165 脂肪间充质干细胞 重组腺病毒载体 脂肪移植 病毒包装 血管生成 成活率 增强型绿色荧光蛋白 组织缺损 

分 类 号：R394.2[医药卫生—医学遗传学]