冠心病患者血浆中microRNA-145表达与冠状动脉侧支循环形成的相关性研究  

Relationship between the plasma expression of microRNA-145 and formation of coronary collateral circulation in patients with coronary artery disease

作　　者：唐义信[1] 孙权[2] 朱灵萍 童霄羽 罗瑛[2] 

机构地区：[1]中南大学湘雅医院心血管内科,长沙410008 [2]中南大学湘雅医院老年病科,长沙410008

出　　处：《中国心血管杂志》2015年第4期262-266,共5页Chinese Journal of Cardiovascular Medicine

基　　金：湖南省科技计划项目(2014sk3014)~~

摘　　要：目的探讨冠心病患者血浆中microRNA-145(miRNA-145)表达与冠状动脉侧支循环(CCC)形成的相关性。方法根据冠状动脉造影结果入选80例主要冠状动脉中至少一支直径狭窄≥90%的冠心病患者,按照Rentrop分级方法对CCC进行分级,其中0级24例,1级19例,2级20例,3级17例。Real-time PCR测定血浆中miRNA-145的相对表达,酶联免疫吸附法检测血浆中血管内皮生长因子(VEGF)水平,Gensini积分方法评价冠状动脉病变程度。结果随着CCC分级的增加,血浆中VEGF水平亦随之升高,miRNA-145相对表达随之下降。控制miRNA-145相对表达水平后,血浆中VEGF水平与CCC的建立呈正相关(r=0.524,P<0.01),控制血浆中VEGF水平后,miRNA-145相对表达与CCC的建立呈负相关(r=-0.669,P<0.01),同时miRNA-145相对表达水平与血浆中VEGF水平呈负相关(r=-0.529,P<0.01)。结论在冠心病患者中,CCC形成良好的患者血浆中miRNA-145相对表达低于CCC形成不良患者,表明miRNA-145在CCC的建立中可能起重要作用。Objective To investigate the relationship between the expression of microRNA-145 （miRNA-ld5） in plasma and the formation of coronary collateral circulation （CCC） in coronary artery disease patients. Methods A total of 80 patients with coronary stenosis were recruited. The CCC degree was rated by Rentrop rating system： rate0 （n =24） , rate 1 （n = 19） , rate2 （n =20） , rate3 （n =17）. Plasma expression of miRNA-145 and concentration of VEGF were measured by real-time PCR and ELISA kit respectively. The severity of coronary artery atherosclerosis was evaluated by Gensini score system. Results The concentrations of VEGF in patients with coronary artery disease were increased according to CCC rating, whereas the expressions of miRNA-145 were decreased according to CCC rating. Correlation analysis demonstrated that there was a positive correlation between the plasma concentration of VEGF and CCC rating （ r = 0. 672, P 〈 0.01 ） ; while the expression of miRNA-145 in plasma showed a significantly negative correlation with the CCC rating and concentration of VEGF in those patients respectively （ r = 0. 761, P 〈 0.01, r = - 0. 529, P 〈 0.01 ）. Conclusions In patients of coronary artery disease with better CCC formation, the expression of miRNA-145 in plasma is significantly lower than those with poor CCC formation, these findings may indicate that miRNA-145 may play an important role in the formation of CCC.

关 键 词：动脉粥样硬化 血管成形术 经腔 经皮冠状动脉 侧支循环 MicroRNA-145 

分 类 号：R541.4[医药卫生—心血管疾病]