乳化异氟醚后处理对在体大鼠心脏缺血-再灌注损伤的保护作用及机制研究  

Emulsified Isoflurane Induces Postconditioning against Myocardial Ischemia and Reperfusion Injury in Rats

作　　者：孔祥云[1] 胡朝阳[1] 刘进[1] 罗朝志[1] 

机构地区：[1]四川大学华西医院麻醉科,成都610041

出　　处：《华西医学》2015年第8期1461-1464,共4页West China Medical Journal

基　　金：国家自然科学基金(30901412)~~

摘　　要：目的研究乳化异氟醚后处理对大鼠心肌缺血再灌注损伤的保护作用,并对其可能的作用机制进行初步探讨。方法 2012年3月-10月采用结扎大鼠左冠状动脉前降支30 min后再灌注180 min,制作大鼠心肌缺血-再灌注损伤模型。在再灌注开始时,尾静脉注射相同容积[4 m L/(kg·h)]的生理盐水,30%脂肪乳,乳化异氟醚30 min。测定心肌梗死面积、脂质过氧化指标和血浆肿瘤坏死因子-α(TNF-α)含量变化,免疫组织化学测量心肌TNF-α蛋白的表达,探讨乳化异氟醚后处理心肌保护的效果和机制。结果再灌注180 min后,与模型组相比,乳化异氟醚显著降低大鼠心肌梗死面积,降低丙二醛含量,升高超氧化物歧化酶活性,降低血浆TNF-α含量和心肌细胞TNF-α蛋白表达。结论后处理乳化异氟醚对大鼠心肌缺血-再灌注损伤具有保护作用,其机制与抑制心肌脂质过氧化和TNF-α有关。Objective To investigate whether emulsified isoflurane applied after an ischemic episode induces postconditioning in an ischemia model of myocardial injury and its underlying mechanism. Methods Between March and October 2012, using a model of in situ myocardial ischemia and reperfusion injury in rats, cardioprotective effects of emulsified isoflurane were examined by determining infarct size, myocardial damage markers and the concentration of tumor necrosis factor （TNF）-α. Results Emulsified isoflurane postconditioning limited infarct size compared with control groups. It increased serum concentrations of superoxide dismutase while decreased malonaldehyde. TNF-α positive cells were also significantly reduced in emulsified isoflurane group compared with control group. Infusion of intralipid had no effect on infarct size or other variables. Conclusion Intravenous administration of emulsified isoflurane after reperfusion protects hearts against reperfusion injury, which may be mediated by the inhibition of cardiac damage markers and the concentration of TNF-α.

关 键 词：乳化异氟醚 后处理 心肌缺血-再灌注损伤 大鼠 肿瘤坏死因子-Α 

分 类 号：R614[医药卫生—麻醉学]