不同灸温对急性佐剂性关节炎大鼠血清IL-1β、IL-2及TNF-α含量的影响  

Effect of Different Temperature Moxibustion on Serum IL-1β, IL-2 and TNF-α Contents in Acute Adjuvant Arthritis Rats

作　　者：季辉[1] 王玲玲[1] 周攀[1] 谢波[1] 

机构地区：[1]南京中医药大学,南京210029

出　　处：《上海针灸杂志》2015年第7期597-599,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家重点基础研究发展计划项目(2009CB522900);江苏省普通高校研究生科研创新计划项目(CXLX13_58)

摘　　要：目的观察不同灸温下艾灸对于急性佐剂性关节炎大鼠血清IL-1IL-1β、IL-2及TNF-α含量的影响,为艾灸的局部抗炎免疫作用机制提供依据。方法采用弗氏完全佐剂(FCA)造模法制备大鼠佐剂性关节炎模型(AA)。将32只SD大鼠随机分出8只作为正常组,其余24只均造模处理,造模成功后随机分为模型组、治疗1组和治疗2组,每组8只。治疗1组和治疗2组均采用灸法治疗,治疗1组将施灸穴位局部温度控制在(38±1)℃,治疗2组将施灸穴位局部温度控制在(45±1)℃。采用酶联免疫法(ELISA)检测大鼠血清IL-1β、IL-2及TNF-α含量。结果模型组、治疗1组和治疗2组血清IL-1β、IL-2及TNF-α含量与正常组比较,差异均具有统计学意义(P<0.01)。治疗1组血清IL-2含量与模型组比较,差异具有统计学意义(P<0.01)。治疗2组血清IL-1β、IL-2及TNF-α含量与模型组和治疗1组比较,差异均具有统计学意义(P<0.01)。结论艾灸具有降低血清中IL-1β、TNF-α含量,提高IL-2含量作用,且45℃灸温能够提高艾灸的作用。艾灸抗炎机制可能是通过降低血清中IL-1β、TNF-α含量,提高IL-2含量来缓解机体炎症反应,且艾灸作用发挥需要适宜的灸温。Objective To investigate the effect of different temperature moxibustion on serum IL-1β, IL-2 and TNF-αcontents in acute adjuvant arthritis rats and provide a basis for the mechanism of local anti-inflammatory and immune action of moxibustion. Methods A rat model of adjuvant arthritis （AA） was made by Freund’s complete adjuvant （FCA） modeling method. Of 32 SD rats, 8 were randomly selected as a normal group and the other 24 for model making. After successful model making, the rats were randomly allocated to model, treatment 1 and treatment 2 groups, 8 rats each. Treatment 1 and 2 groups received moxibustion. Local temperature at moxibustion point was controlled at （38±1）℃in treatment 1 group and at （45±1）℃in treatment 2 group. Rat serum IL-1β, IL-2 and TNF-αcontents were measured by enzyme-linked immunosorbent assay （ELISA）. Results There were statistically significant differences in serum IL-1β, IL-2 and TNF-αcontents between the model, treatment 1 or treatment 2 group and the normal group （P〈0.01）. There was a statistically significant difference in serum IL-2 content between treatment 1 and the model groups （P〈0.01）. There were statistically significant differences in serum IL-1β, IL-2 and TNF-αcontents between treatment 2 group and the model or treatment 1 group （P〈0.01）. Conclusions Moxibustion has a reducing effect on serum IL-1βand TNF-αcontents and a raising effect on IL-2 content. 45℃moxibustion temperature can improve the effect of moxi bustion. The anti-inflammatory action of moxibustion may be through the mechanism of reducing serum IL-1βand TNF-αcontents and raising IL-2 content, which relieve body inflammatory reaction. The action of moxibustion needs proper moxibustion temperature.

关 键 词：针灸疗法 灸法 灸温 佐剂性关节炎 细胞因子 大鼠 IL-1Β IL-2 TNF-Α 

分 类 号：R2-03[医药卫生—中医学]