电针对功能性消化不良大鼠下丘脑和海马胃促生长素及受体mRNA表达的影响  

Effect of Electroacupuncture on Hypothalamic and Hippocampal Expressions of Ghrelin and Ghrelin Receptor m RNA in Functional Dyspepsia Rats

作　　者：周利[1] 程艳萍[1] 

机构地区：[1]湖北中医药大学,武汉430060

出　　处：《上海针灸杂志》2015年第7期673-677,共5页Shanghai Journal of Acupuncture and Moxibustion

基　　金：武汉市卫计委2014年度医疗卫生科研基金(WZ14B02)

摘　　要：目的观察电针对功能性消化不良(FD)大鼠下丘脑和海马胃促生长素(Ghrelin)及Ghrelin受体(GHS-R)m RNA表达的影响。方法将80只SD大鼠随机分为正常对照组、模型对照组、药物治疗组和电针治疗组,每组20只。采用夹尾造模法复制FD大鼠模型,药物治疗组采用西沙必利溶液灌胃治疗,电针治疗组采用电针治疗。4组均采用Western blot法检测下丘脑和海马Ghrelin蛋白水平的表达,Real-time PCR法检测下丘脑和海马GHS-R m RNA的表达。结果与模型对照组比较,药物治疗组大鼠下丘脑Ghrelin蛋白、下丘脑、海马GHS-R m RNA的表达均升高(P<0.05),电针治疗组大鼠下丘脑、海马Ghrelin蛋白GHS-R m RNA的表达上调(P<0.05);与药物治疗组比较,电针治疗组大鼠海马Ghrelin蛋白和GHS-R m RNA的表达上调(P<0.05)。结论电针和药物可通过调节FD大鼠下丘脑和海马Ghrelin蛋白和GHS-R m RNA的含量,激活下丘脑神经元和海马兴奋性突触,通过海马-下丘脑通路,发挥胃肠效应,良性调节脑肠轴的失衡状态。Objective To investigate the effect of electroacupuncture on hypothalamic and hippocampal expressions of ghrelin and ghrelin receptor （GHS-R） mRNA in functional dyspepsia （FD） rats. Methods Eighty SD rats were randomly allocated to normal control, model control, medication and electroacupuncture groups, 20 rats each. A rat model of FD was made by tail clamp modeling method. The medication group received an oral gavage of cisapride solution and the electroacupuncture group, electroacupuncture treatment. The expressions of ghrelin protein and GHS-R mRNA in the hypothalamus and hippocampus were examined by Western blot and real-time PCR, respectively, in all the four groups. Results Compared with the model control group of rats, the expression of ghrelin protein in the hypothalamus and the expression of GHS-R mRNA in the hypothalamus and hippocampus increased in the medication group （P〈0.05） and the expressions of ghrelin protein and GHS-R mRNA in the hypothalamus and hippocampus were up-regulated in the electroacupuncture group （P〈0.05）. Compared with the medication group of rats, the expressions of ghrelin protein and GHS-R mRNA in the hippocampus were up-regulated in the electroacupuncture group （P〈0.05）. Conclusions Electroacupuncture and medication can improve gastrointestinal functions and correct an imbalance in the brain-gut axis through the hippocampus-hypothalamic pathway by regulating hypothalamic and hippocampal ghrelin protein and GHS-R mRNA contents and activating hypothalamic neurons and hippocampal excitatory synapses.

关 键 词：针刺疗法 电针 消化不良 功能性 GHRELIN GHS-R 大鼠 

分 类 号：R203[医药卫生—中医学]