HBsAg阳性母亲新生儿外周血IL-10和IL-12水平与乙型肝炎疫苗免疫应答的关系  

Relationship between IL-10 and IL-12 levels in neonatal peripheral blood and responsiveness to hepatitis B vaccine of infants born to HBs Ag positive mothers

作　　者：王婷[1] 高怡[1] 温海秀 郭健[1] 汪波[2] 丰淑英 王素萍[1] 

机构地区：[1]山西医科大学公共卫生学院流行病学教研室,山西太原030001 [2]山西省太原市第三人民医院妇产科,山西太原030001

出　　处：《中华疾病控制杂志》2015年第8期765-768,786,共5页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金(81072341)

摘　　要：目的 探讨乙型肝炎病毒表面抗原（hepatitis B surface antigen,HBsAg）阳性母亲新生儿外周血白细胞介素-10（interleukin-10,IL-10）和白细胞介素-12（interleukin-12,IL-12）水平与其接种乙肝疫苗无、弱应答的关系。方法 选择98对HBsAg阳性母亲及其足月分娩新生儿,采用荧光定量聚合酶链反应（fluorescent quantitation poly-merase chain reaction,FQ-PCR）和化学发光免疫试验（chemiluminescence immunoassay,CLIA）分别检测母儿乙型肝炎病毒脱氧核糖核酸（hepatitis B virus deoxyribonucleic acid,HBVDNA）和HBV血清学标志物,酶联免疫吸附试验（en-zyme-linked immunosorbant assay,ELISA）检测新生儿外周血IL-10、IL-12水平。新生儿全程接种乙肝疫苗随访至12月龄,检测血清抗-HBs水平。结果 乙肝疫苗无/弱应答率为32.65%。单因素分析,新生儿外周血IL-10和IL-12水平与乙肝疫苗免疫应答有关（均有P〈0.05）。非条件Logistic回归模型调整母亲HBeAg、母亲HBVDNA、新生儿乙型肝炎e抗原（hepatitis Beantigen,HBeAg）等因素,新生儿外周血IL-10水平〈174.05pg/ml者易发生乙肝疫苗免疫无或弱应答（OR=8.35,95%CI：1.83-38.14）。分级分析,乙肝疫苗无/弱应答率随IL-10水平增加呈下降趋势（2趋势=4.41,P=0.036）。结论 HBsAg阳性母亲婴儿乙肝疫苗免疫无或弱应答可能与其新生儿期外周血IL-10水平低下有关。Objective To investigate the relationship between the level of interleukin-10（ IL-10） and interleukin-12（ IL-12） in neonatal peripheral blood and non-/ low-responsiveness to hepatitis B vaccine of infants born to HBs Ag positive mothers. Methods Ninety-eight HBs Ag positive mothers and their full-term neonates were enrolled. Maternal and neonatal hepatitis B virus（ HBV） DNA and HBV serologic markers were measured using chemiluminescence immunoassay（ CLIA） and fluorescence quantitative polymerase chain reaction（ FQ-PCR）,respectively. The level of IL-10 and IL-12 in neonatal peripheral blood were determined by enzyme-linked immunosorbant assay（ ELISA）. These neonates were followed up at the age of one year,and the anti-HB titer of these infants were determined by CLIA. Results The rate of non-/ lowresponsiveness was 32. 65%. In univariate analysis,the level of IL-10 and IL-12 in neonatal peripheral blood was significantly associated with responsiveness to hepatitis B vaccine（ all P〈0. 05）. Maternal HBe Ag,maternal HBV DNA,neonatal hepatitis B e antigen（ HBe Ag） and other factors were adjusted by unconditional logistic regression model. The result revealed that when the level of IL-10 in neonatal peripheral blood was less than 174. 05 pg / ml,the infants were more likely to be non-/ low-responsive to hepatitis B vaccine（ OR = 8. 35,95% CI： 1. 83-38. 14）. Classification analysis showed that the rate of non-/ low-responsive was negatively associated with the level of IL-10（χ2trend= 4. 41,P = 0. 036）. Conclusions Infants born to HBs Ag positive mothers with lower level of IL-10 in neonatal peripheral blood were more likely to be non-/low-responsive to hepatitis B vaccine.

关 键 词：婴儿 新生 肝炎 乙型 病例对照研究 

分 类 号：R181[医药卫生—流行病学]