二茂铁功能化石墨烯修饰电极用于多巴胺的检测  

Preparation of Ferrocene Functionalized Graphene Modified Electrode and Its Application in Detection of Dopamine

作　　者：刘超[1] 何建新[1] 古宁宇[1] 蔡建信[2] 王小磊[1] 杨震宇[1] 

机构地区：[1]南昌大学化学学院,南昌330031 [2]南昌大学资源环境与化工学院,南昌330031

出　　处：《分析化学》2015年第8期1175-1180,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21363015;21263016;21461015);江西省高校科技落地计划(No.KJLD14010)资助~~

摘　　要：通过Friedel-Crafts酰基化反应制备二茂铁(Fc)功能化氧化石墨烯材料,利用透射电镜、傅里叶变换红外光谱和X-射线衍射对该复合物的形貌和结构进行了表征。将其滴涂到玻碳电极(GCE)表面并用Nafion膜固定,进行电化学还原,得到电化学还原氧化石墨烯复合材料修饰电极(Fc/ERGO/GCE)。此修饰电极对多巴胺的氧化还原具有良好的催化性能,在循环伏安曲线的0.2 V附近出现一对多巴胺的可逆氧化还原峰。差示扫描伏安法(DPV)峰电流大小与多巴胺浓度在0.4~300μmol/L范围内呈良好的线性关系(R2=0.9998),检出限为0.08μmol/L。用于检测人体尿液中的多巴胺含量,加标样品的回收率在92.5%~105.0%之间,相对标准偏差小于5%(n=5),表明制备的Fc/ERGO/GCE可用于实际样品中DA的检测。A complex of ferrocene functionalized graphene oxide （Fc/GO） was prepared through Friedel- Crafts acylation method and characterized by tunable electron microscopy （TEM）, Fourier transform infrared spectroscopy （FTIR） and X-ray diffraction （XRD）. The Fc/GO complex was immobilized on glassy carbon electrode （GCE） with Nation, and electrochemically reduced by potentiostatic reduction method to get Fc/ ERGO/GCE. The results showed that the obtained Fc/ERGO/GCE had good catalytic performance for the oxidation-reduction of dopamine （DA）. A pair of peaks near 0.2 V in the cyclic vohammogram attributed to DA was observed. The differential pulse vohammetric （DPV） peak current was proportional to the concentration of DA in the range from 0.4μmol/L to 300 μmol/L （ R2 = 0. 9998 ）, with a limit of detection of 0.08 p^mol/L. The recoveries of DA in the spiked human urine samples were 92.5%-105.0% with relative standard derivations （RSDs） of less than 5% （ n = 5 ）, which indicated that the fabricated Fc/ERGO/GCE was suitable for the determination of dopamine in real samples.

关 键 词：二茂铁 电化学还原氧化石墨烯 多巴胺 电化学检测 

分 类 号：O657.92[理学—分析化学] R927[理学—化学]