电针对脑缺血再灌注大鼠纹状体D1R和DAT表达的影响  

Effect of Electroacupuncture on Striatal D1R and DAT Expressions in Cerebral Ischemia-reperfusion Rats

作　　者：徐鸣曙[1] 陈春艳 李昌植[1] 葛林宝 张淑静[1] 赵丹[2] 李明哲[1] 韩清[3] 张英杰[3] 

机构地区：[1]上海市针灸经络研究所,上海200030 [2]上海市气功研究所,上海200030 [3]上海中医药大学,上海201203

出　　处：《上海针灸杂志》2015年第8期791-793,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81001547);上海市重点学科建设项目(S30304);上海中医药大学预算内项目(2014YSN55);上海市卫生局"海派中医流派传承研究基地"项目(ZYSNXD-CCHPGC-JD-014);上海高校青年教师培养资助计划(SZY13052)

摘　　要：目的探讨电针、多巴胺D1受体(D1R)工具药及针药结合对大脑中动脉缺血(MCAO)再灌注大鼠纹状体内D1R和多巴胺转运体(DAT)表达的影响。方法将47只SD雄性大鼠随机分组、造模、施予相应干预措施,6 h后取材固定,用免疫组化法检测不同组别纹状体内D1R、DAT的表达。结果模型组神经功能缺血评分明显高于空白组,电针治疗可使评分明显降低(P<0.05)。拮抗剂组、电针组、电针+拮抗剂组D1R表达与模型组比较显著下调(P<0.05);模型组D1R表达与激动剂组、电针+激动剂组比较无显著变化(P>0.05);各组DAT表达与模型组比较均显著下调(P<0.05),除模型组外各组间比较有差异,但无统计学意义(P>0.05)。结论脑缺血再灌注可导致大鼠缺血侧纹状体内D1R和DAT表达的增高;电针、D1R拮抗剂及二者结合可明显下调D1R的表达,具有脑保护作用;电针与D1R拮抗剂的效应不能叠加,D1R信号通路可能是电针发挥脑保护作用的途径之一。Objective To investigate the effects of dopamine D1 receptor（D1R） tool drugs and combined acupuncture and medicine on striatal expressions of D1 R and dopamine transporters（DAT） in middle cerebral artery occlusion（MCAO）-reperfusion rats. Method Forty-seven male SD rats were randomly grouped, used to make a model and given corresponding interventions. The materials were taken and fixed six hrs later. Striatal D1 R and DAT expressions were detected by an immunohistochemical method in different groups. Result The neurological deficit score was significantly higher in the model group than in the blank group. Electroacupuncture treatment decreased the score significantly（P〈0.05）. D1 R expression was significantly down-regulated in the antagonist, electroacupuncture and electroacupuncture plus antagonist groups compared with the model group（P〈0.05）. There was no statistically significant difference in D1 R expression between the model group and the antagonist or electroacupuncture plus antagonist group（P〉0.05）. DAT expression was significantly down-regulated in the other groups compared with the model group（P〈0.05）. There was no statistically significant difference in D1 R expression between all groups except the model group（P〉0.05）. Conclusion Cerebral ischemia-reperfusion can result in high D1 R and DAT expressions in rat striatum on the ischemic side. Electroacupuncture, D1 R antagonists and a combination of the two can significantly down-regulate D1 R expression and have a protective effect on the brain. The effects of electroacupuncture and D1 R antagonists can not be added to each other. D1 R signaling pathway may be one of ways by which electroacupuncture produces a protective effect on the brain.

关 键 词：电针 脑缺血再灌注 多巴胺D1受体 多巴胺转运体 免疫组化 大鼠 

分 类 号：R2-03[医药卫生—中医学]