铟对人外周血淋巴细胞微核形成的影响  

Effects of indium on micronucleus formation in human peripheral blood lymphocytes

作　　者：郭妍 惠长野 张镏琢 王丽丽[2] 王佃鹏 杨学琴 杨新跃 李智民 

机构地区：[1]深圳市职业病防治院国家职业病临床重点专科,518001 [2]河北北方学院医学检验学院

出　　处：《中华劳动卫生职业病杂志》2015年第8期563-565,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的探讨氯化铟（InCl3）对体外培养的人原代淋巴细胞的细胞毒性、微核形成的影响。方法以体外培养的淋巴细胞为研究材料，采用CCK-8法观察不同浓度氯化铟（4、40、80、200、500、1000umol／L）染毒24h对淋巴细胞的毒性作用；采用胞质分裂阻断法检测不同浓度氯化铟染毒淋巴细胞的微核水平及抗氧化剂维生素C对微核率的影响。结果氯化铟染毒浓度≥500μmol／L，淋巴细胞存活率低于对照组，差异有统计学意义（P〈0．05）；80μmol／L氯化铟染毒可导致纯培养淋巴细胞微核率明显上升，差异有统计学意义（P〈0．05）；而氯化铟浓度为200μmol／L，微核率未进一步上升；500和1000μmol／L氯化铟染毒可引起全血培养体系淋巴细胞微核率显著升高，差异有统计学意义（P〈0．001）；20和100μmol／L维生素C可部分拮抗染氯化铟引起的淋巴细胞微率升高。结论氯化铟可导致体外培养的人原代淋巴细胞微核率升高，可能与氧化应激引起的DNA损伤有关。Objective To investigate the cytotoxicity of indium chloride （InCl3） and its effects on micro- nucleus formation in primary human lymphocytes cultured in vitro. Methods The CCK-8 assay was used to evaluate the cytotoxicity of 24 h exposure to different concentrations of InCl3 （4, 40, 80, 200, 500, and 1 000μmol/L） in lymphocytes cultured in vitro. The cytokinesis-block method was used to determine the micronucleus level in lymphocytes exposed to different concentrations of InC13 and the effects of anti-oxidant vitamin C on micronucleus frequency. Results Lymphocytes exposed to InCl3 of no less than 500 μmol/L had significantly lower survival rates than those in the control group （P〈0.05）. Lymphocytes exposed to 80 μmol/L InCl3 had a significantly higher micronucleus frequency than those in the control group （P〈0.05）. However, there was no further increase in micronucleus frequency of lymphocytes exposed to 200μmol/L InCl3. Lymphocytes cultured in whole blood and exposed to 500 or 1000 μmol/L InCl3 had a significantly increased micronucleus frequency than those in the control group （P〈0.001）. The increase in micronucleus frequency of lymphocytes induced by indium could be partially antagonized by 20 or 100 μmol/L vitamin C. Conclusion InCl3 can induce an increase in micronucleus frequency of primary human lymphocytes cultured in vitro, which might be associated with DNA damage induced by oxidative stress.

关 键 词：铟 淋巴细胞 遗传毒性 微核 

分 类 号：R135[医药卫生—劳动卫生]