尿hepcidin-25检测在儿童铁缺乏症早期诊断中的应用  

Application of urinary hepcidin-25 determination in the early-stage diagnosis of iron deficient syndrome among children

作　　者：徐霜清[1] 邹小凤[1] 马国祥 徐克前[3] 

机构地区：[1]广州金域医学检验中心,广东广州510330 [2]深圳福永人民医院,广东深圳518103 [3]中南大学湘雅医学院医学检验系,湖南长沙410000

出　　处：《检验医学》2015年第8期777-781,共5页Laboratory Medicine

摘　　要：目的初步研究尿hepcidin-25水平在儿童铁缺乏症(IDS)早期诊断中的诊断性能。方法采用酶联免疫吸附试验(ELISA)检测25例贮存铁耗尽(ID)阶段患儿、25例缺铁性红细胞生成(IDE)阶段患儿、25例缺铁性贫血(IDA)患儿和25名健康儿童(正常对照组)尿hepcidin-25浓度。采用受试者工作特征(ROC)曲线确定尿hepcidin-25诊断ID、IDE、IDA的临界值及其相应的诊断性能。结果 ID组、IDE组、IDA组及正常对照组两两之间尿hepcidin-25水平差异均有统计学意义(P均<0.05)。尿hepcidin-25对鉴别正常对照组与ID组、IDE组、IDA组的ROC曲线下面积分别为0.865、0.974、0.998;鉴别IDE组与IDA组、ID组、正常对照和ID混合组的ROC曲线下面积分别为0.872、0.870、0.922。结论尿hepcidin-25检测是一项简单而没有创伤的试验,在儿童IDS的早期诊断中具有一定的应用价值。Objective To study the diagnostic performance of urinary hepcidin-25 for the early-stage diagnosis of iron deficient syndrome （IDS） among children. Methods By enzyme-linked immunosorbent assay （ELISA）, urinary hepcidin-25 levels were determined in 25 cases of iron depletion （ID） stage, 25 cases of iron deficient erythropoiesis （IDE） stage, 25 cases of iron deficient anemia（IDA） and 25 healthy children （control group）. The cut-off values of urinary hepcidin-25 for ID, IDE and IDA and corresponding diagnostic performance were analyzed by receiver operating characteristic （ROC） curve. Results There was statistical significance of urinary hepcidin-25 levels in comparison between any 2 groups of ID, IDE, IDA and control groups （ P 〈 0.05 ）. The areas under ROC curves for urinary hepcidin-25 for differentiating ID, IDE and IDA groups from control group were 0.865, 0. 974 and 0.998, respectively. The areas under ROC curves for urinary hepcidin-25 for differentiating IDE from IDA, differentiating IDE from ID, differentiating IDE from the mixing group of control with ID were 0. 872, 0. 870 and 0. 922, respectively. Conclusions The determination of urinary hepcidin-25 level is a simple and non-invasive test, and it might have application significance in the early-stage diagnosis of IDS among children.

关 键 词：hepcidin-25 铁缺乏 诊断试验 性能 

分 类 号：R446.1[医药卫生—诊断学]