响应曲面法验证淀粉酶检测程序最适pH值、Cl^-和EPS浓度  

Response surface method to verify amylase determination procedure's optimum pH,chloride ion and EPS concentrations

作　　者：孟舒婷 王惠民[1] 季伙燕[1] 王建新[1] 许丽丽[1] 袁若愚 顾文超[1] 

机构地区：[1]南通大学附属医院,江苏南通226001

出　　处：《检验医学》2015年第8期782-786,共5页Laboratory Medicine

摘　　要：目的证明响应曲面法(RSM)是确认淀粉酶(AMY)参考测量程序最佳测量条件的方法。方法采用单因素方差分析研究pH值、氯离子(Cl-)、亚乙基-P-硝基苯-D-麦芽七糖苷(EPS)浓度对AMY活性的影响是否具有统计学意义。采用Box-Behnken试验设计,在实验室条件下研究pH值(6.9~7.1)、Cl-(50~90 mmol/L)和EPS浓度(4.5~5.5 mmol/L)对AMY活性的联合效应,建立pH值、Cl-和EPS对AMY活性影响的定量关系模型,优化得出pH值、Cl-和EPS的最佳组合。结果 pH值、Cl-和EPS对AMY活性影响有统计学意义(P<0.05);pH值、Cl-和EPS对AMY活性影响的一次、二次效应均有统计学意义(P<0.01),Cl-和EPS交互作用有统计学意义(P<0.05)。AMY最优条件:pH值7.02、Cl-72.79 mmol/L、EPS 5.07 mmol/L。结论所得pH值、Cl-和EPS的最佳测量条件与国际临床化学和检验医学联合会(IFCC)推荐条件无差异,RSM可以作为确认参考测量程序最佳测量条件的方法。Objective To confirm that response surface method （RSM） is a method for amylase （AMY） reference determination procedure with optimum conditions. Methods The single factor analysis of variance was used to study pH, chloride ion （Cl^-） and ethylidene-4-nitrophenyl-alpha-D-maltoheptaoside （EPS） concentrations in order to know whether they had statistical significance for AMY activity. Box-Behnken design was used to study the combined influence of pH （6.9-7.1）, Cl^- （50-90 mmol/L） and EPS （4.5-5.5 mmol/L） concentrations on AMY activity. The quantitative relation model on pH, Cl^- and EPS concentrations for AMY activity was established in order to obtain their optimum combination. Results There was statistical significance on the influence of the 3 factors on AMY activity （ P 〈 0.05 ）. The one-shot and second-order effects of pH, Cl^-and EPS had statistical significance on AMY activity （P 〈 0.01 ）. Cl^- and EPS interacted significantly （ P 〈 0.05 ）. The optimum conditions for AMY were pH 7.02, Cl^- 72.79 mmol/L and EPS 5.07 mmo]/L. Conclusions The optimum conditions of pH, Cl^- and EPS have no difference with those recommended from the International Federation of Clinical Chemistry and Laboratory Medicine （IFCC）. RSM is a method with optimum conditions to confirm the reference determination procedure.

关 键 词：响应曲面法 淀粉酶 单因素方差分析 pH值 氯离子 亚乙基-P-硝基苯-D-麦芽七糖苷 

分 类 号：R446.1[医药卫生—诊断学]